149891 20251017144636.0 doi 10.1016/j.xpro.2023.102730 sideral 137058 ART-2023-137058 eng García-Mendívil, Laura Universidad de Zaragoza (orcid)0000-0002-2954-1068 Automatic quantification of myocardial remodeling features in human ventricular tissue from label-free microscopy 2023 Access copy available to the general public Unrestricted The procedures used routinely for collagen and lipofuscin evaluation are, in many cases, qualitative, observer dependent, and disregard spatial distribution. Here, we present a protocol for automatic quantification and spatial characterization of collagen and lipofuscin from label-free microscopy images of human ventricular tissues. We describe the steps for sample collection, tissue processing, image acquisition, and quantification of collagen and lipofuscin. This protocol avoids discrepancies between observers and can be adapted to other tissues and species. For complete details on the use and execution of this protocol, please refer to García-Mendívil et al. (2022). info:eu-repo/semantics/openAccess by-nc-nd https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es 0.955 2023 Immunology and Microbiology (miscellaneous) 2023 Q1 Neuroscience (miscellaneous) 2023 Q2 Biochemistry, Genetics and Molecular Biology (miscellaneous) 2023 Q2 2.0 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pérez-Zabalza, María (orcid)0000-0002-3194-7796 Duwé, Sam Ordovás, Laura (orcid)0000-0003-3982-1263 Pueyo, Esther Universidad de Zaragoza (orcid)0000-0002-1960-407X 5008 800 Universidad de Zaragoza Dpto. Ingeniería Electrón.Com. Área Teoría Señal y Comunicac. 4, 4 (2023), 102730 [14 pp.] STAR Protocols 2666-1667 4611391 http://zaguan.unizar.es/record/149891/files/texto_completo.pdf Versión publicada 1487216 http://zaguan.unizar.es/record/149891/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:149891 articulos driver 2025-10-17-14:29:17 ARTICLE