150248 20251017144616.0 doi 10.1016/j.ijpharm.2021.121014 sideral 126066 ART-2021-126066 eng Paz-Artigas, L Universidad de Zaragoza (orcid)0000-0001-6139-5905 Benefits of cryopreservation as long-term storage method of encapsulated cardiosphere-derived cells for cardiac therapy: A biomechanical analysis 2021 Cardiosphere-derived cells (CDCs) encapsulated within alginate-poly-L-lysine-alginate (APA) microcapsules present a promising treatment alternative for myocardial infarction. However, clinical translatability of encapsulated CDCs requires robust long-term preservation of microcapsule and cell stability, since cell culture at 37 degrees C for long periods prior to patient implantation involve high resource, space and manpower costs, sometimes unaffordable for clinical facilities. Cryopreservation in liquid nitrogen is a well-established procedure to easily store cells with good recovery rate, but its effects on encapsulated cells are understudied. In this work, we assess both the biological response of CDCs and the mechanical stability of microcapsules after long-term (i.e., 60 days) cryopreservation and compare them to encapsulated CDCs cultured at 37 degrees C. We investigate for the first time the effects of cryopreservation on stiffness and topographical features of microcapsules for cell therapy. Our results show that functionality of encapsulated CDCs is optimum during 7 days at 37 degrees C, while cryopreservation seems to better guarantee the stability of both CDCs and APA microcapsules properties during longer storage than 15 days. These results point out cryopreservation as a suitable technique for long-term storage of encapsulated cells to be translated from the bench to the clinic. info:eu-repo/grantAgreement/EC/H2020/78132/EU/Electronic Components and Systems for European Leadership Joint Undertaking/ECSEL JU-78132-Position-II-2017-IA This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 78132-ECSEL JU-78132-Position-II-2017-IA info:eu-repo/semantics/closedAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 6.51 2021 PHARMACOLOGY & PHARMACY 40 / 279 = 0.143 2021 Q1 T1 1.0 2021 Pharmaceutical Science 2021 Q1 9.6 2021 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Ziani, K Alcaine, C Universidad de Zaragoza (orcid)0000-0002-7854-8856 Baez-Diaz, C Blanco-Blazquez, V Pedraz, JL Ochoa, I Universidad de Zaragoza (orcid)0000-0003-2410-5678 Ciriza, J Universidad de Zaragoza (orcid)0000-0002-8666-622X 1006 255 Universidad de Zaragoza Dpto. Fisiatría y Enfermería Área Enfermería 1003 443 Universidad de Zaragoza Dpto. Anatom.Histolog.Humanas Area Histología 607 (2021), 121014 [10 pp] Int. j. pharm. International Journal of Pharmaceutics 0378-5173 5604064 http://zaguan.unizar.es/record/150248/files/texto_completo.pdf Versión publicada 2454259 http://zaguan.unizar.es/record/150248/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:150248 articulos driver 2025-10-17-14:19:17 ARTICLE