000150345 001__ 150345
000150345 005__ 20250203110925.0
000150345 0247_ $$2doi$$a10.1016/bs.mcb.2024.05.009
000150345 0248_ $$2sideral$$a142442
000150345 037__ $$aART-2024-142442
000150345 041__ $$aeng
000150345 100__ $$aBeltrán-Visiedo, Manuel
000150345 245__ $$aCytofluorometric assessment of calreticulin exposure on CD38+ plasma cells from the human bone marrow
000150345 260__ $$c2024
000150345 5060_ $$aAccess copy available to the general public$$fUnrestricted
000150345 5203_ $$aExposure of the endoplasmic reticulum chaperone calreticulin (CALR) on the surface of stressed and dying cells is paramount for their effective engulfment by professional antigen-presenting cells such as dendritic cells (DCs). Importantly, this is required (but not sufficient) for DCs to initiate an adaptive immune response that culminates with an effector phase as well as with the establishment of immunological memory. Conversely, the early exposure of phosphatidylserine (PS) on the outer layer of the plasma membrane is generally associated with the rapid engulfment of stressed and dying cells by tolerogenic macrophages. Supporting the clinical relevance of the CALR exposure pathway, the spontaneous or therapy-driven translocation of CALR to the surface of malignant cells, as well as intracellular biomarkers thereof, have been associated with improved disease outcome in patients affected by a variety of neoplasms, with the notable exception of multiple myeloma (MM). Here, we describe an optimized protocol for the flow cytometry-assisted quantification of surface-exposed CALR and PS on CD38+ plasma cells from the bone marrow of patients with MM. With some variations, we expect this method to be straightforwardly adaptable to the detection of CALR and PS on the surface of cancer cells isolated from patients with neoplasms other than MM.
000150345 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000150345 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000150345 700__ $$aSerrano-Del Valle, Alfonso
000150345 700__ $$aJiménez-Aldúan, Nelia
000150345 700__ $$aSoler-Agesta, Ruth$$uUniversidad de Zaragoza
000150345 700__ $$0(orcid)0000-0003-2156-8378$$aNaval, Javier$$uUniversidad de Zaragoza
000150345 700__ $$aGalluzzi, Lorenzo
000150345 700__ $$0(orcid)0000-0002-2315-9079$$aMarzo, Isabel$$uUniversidad de Zaragoza
000150345 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000150345 7102_ $$11002$$2050$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Biología Celular
000150345 773__ $$g189 (2024), 189-206$$pMethods cell biol.$$tMETHODS IN CELL BIOLOGY$$x0091-679X
000150345 8564_ $$s997721$$uhttps://zaguan.unizar.es/record/150345/files/texto_completo.pdf$$yVersión publicada
000150345 8564_ $$s854024$$uhttps://zaguan.unizar.es/record/150345/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000150345 909CO $$ooai:zaguan.unizar.es:150345$$particulos$$pdriver
000150345 951__ $$a2025-02-03-10:50:03
000150345 980__ $$aARTICLE