150345 20260112133358.0 doi 10.1016/bs.mcb.2024.05.009 sideral 142442 ART-2024-142442 eng Beltrán-Visiedo, Manuel Cytofluorometric assessment of calreticulin exposure on CD38+ plasma cells from the human bone marrow 2024 Access copy available to the general public Unrestricted Exposure of the endoplasmic reticulum chaperone calreticulin (CALR) on the surface of stressed and dying cells is paramount for their effective engulfment by professional antigen-presenting cells such as dendritic cells (DCs). Importantly, this is required (but not sufficient) for DCs to initiate an adaptive immune response that culminates with an effector phase as well as with the establishment of immunological memory. Conversely, the early exposure of phosphatidylserine (PS) on the outer layer of the plasma membrane is generally associated with the rapid engulfment of stressed and dying cells by tolerogenic macrophages. Supporting the clinical relevance of the CALR exposure pathway, the spontaneous or therapy-driven translocation of CALR to the surface of malignant cells, as well as intracellular biomarkers thereof, have been associated with improved disease outcome in patients affected by a variety of neoplasms, with the notable exception of multiple myeloma (MM). Here, we describe an optimized protocol for the flow cytometry-assisted quantification of surface-exposed CALR and PS on CD38+ plasma cells from the bone marrow of patients with MM. With some variations, we expect this method to be straightforwardly adaptable to the detection of CALR and PS on the surface of cancer cells isolated from patients with neoplasms other than MM. info:eu-repo/semantics/openAccess by-nc-nd https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es 0.296 2024 Cell Biology 2024 Q4 2.2 2024 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Serrano-Del Valle, Alfonso Jiménez-Aldúan, Nelia Soler-Agesta, Ruth Universidad de Zaragoza Naval, Javier Universidad de Zaragoza (orcid)0000-0003-2156-8378 Galluzzi, Lorenzo Marzo, Isabel Universidad de Zaragoza (orcid)0000-0002-2315-9079 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 1002 050 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Biología Celular 189 (2024), 189-206 Methods cell biol. METHODS IN CELL BIOLOGY 0091-679X 997721 http://zaguan.unizar.es/record/150345/files/texto_completo.pdf Versión publicada 854024 http://zaguan.unizar.es/record/150345/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:150345 articulos driver 2026-01-12-13:22:25 ARTICLE