000150501 001__ 150501
000150501 005__ 20251115074511.0
000150501 0247_ $$2doi$$a10.1007/s00723-024-01725-0
000150501 0248_ $$2sideral$$a142650
000150501 037__ $$aART-2025-142650
000150501 041__ $$aeng
000150501 100__ $$aBracci, Maruan
000150501 245__ $$aPerformance Comparison of Different Rapid Freeze–Quench Strategies for Electron Paramagnetic Resonance
000150501 260__ $$c2025
000150501 5203_ $$aThis work addresses the development of a custom-made home-built rapid freeze–quench (RFQ) device and the comparison of its performance to the one of a commercial RFQ setup that was in-house custom adapted. Both systems consist of two syringes that push the reactants into a mixing chamber and the products to a subsequent freezing setup. Using the binding of azide to myoglobin as a calibration reaction, the quenching times of the different setups were compared, evaluating different instrumental parameters, such as software-controlled variation of the aging time, variations of the flow rate and variations of the distance travelled by the mixed sample before freezing. In addition to minimal sample consumption, the home-built RFQ device was found to lead to the shorter reaction times which could be controlled in a time range from 10 to 25 ms. The commercial RFQ system yielded optimal reaction control in a time range from 50 to 200 ms, although a larger volume of reactants needed to be used due to the significant dead volume of the system. Three different freezing methods were also evaluated, among which, in our hands, freezing the jet directly in a deep bath of cold isopentane yielded shorter and reproducible freezing times.
000150501 536__ $$9info:eu-repo/grantAgreement/ES/AEI/CEX2023-001286-S$$9info:eu-repo/grantAgreement/EC/H2020/813209/EU/Paramagnetic Species in Catalysis Research. A Unified Approach Towards Heterogeneous, Homogeneous and Enzyme Catalysis/PARACAT$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 813209-PARACAT$$9info:eu-repo/grantAgreement/ES/MICINN-AEI/PRTR-C17.I1$$9info:eu-repo/grantAgreement/ES/MICINN/PID2021-127287NB-I00
000150501 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000150501 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000150501 700__ $$aSerra, Ilenia
000150501 700__ $$0(orcid)0000-0002-1827-1250$$aGarcía-Rubio, Inés
000150501 700__ $$aVan Doorslaer, Sabine
000150501 773__ $$g56, 1-2 (2025), 229-252$$pAppl. magn. reson.$$tAPPLIED MAGNETIC RESONANCE$$x0937-9347
000150501 8564_ $$s1689953$$uhttps://zaguan.unizar.es/record/150501/files/texto_completo.pdf$$yVersión publicada$$zinfo:eu-repo/date/embargoEnd/2025-11-15
000150501 8564_ $$s1332275$$uhttps://zaguan.unizar.es/record/150501/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada$$zinfo:eu-repo/date/embargoEnd/2025-11-15
000150501 909CO $$ooai:zaguan.unizar.es:150501$$particulos$$pdriver
000150501 951__ $$a2025-10-17-14:31:20
000150501 980__ $$aARTICLE