000150504 001__ 150504 000150504 005__ 20250411150809.0 000150504 0247_ $$2doi$$a10.1002/smll.202409229 000150504 0248_ $$2sideral$$a142583 000150504 037__ $$aART-2025-142583 000150504 041__ $$aeng 000150504 100__ $$0(orcid)0000-0001-6786-2041$$aBayona, Clara 000150504 245__ $$aA Novel Multicompartment Barrier-Free Microfluidic Device Reveals the Impact of Extracellular Matrix Stiffening and Temozolomide on Immune-Tumor Interactions in Glioblastoma 000150504 260__ $$c2025 000150504 5203_ $$aThe immune system plays a crucial role in shaping the glioblastoma tumor microenvironment, characterized by its complexity and dynamic interactions. Understanding the tumor-immune crosstalk is essential for advancing cancer research and therapeutic development. Here, a novel multicompartment, barrier-free microfluidic device is presented that overcomes the limitations of existing models by enabling direct tumor-immune interactions without physical barriers, preserving natural immune cell infiltration. This platform supports the independent and simultaneous culture of tumor and immune cells, replicating the healthy-tumoral stroma interface, and allows investigating the effect of matrix stiffness and chemotherapy on both populations. The findings reveal that increased collagen concentration promotes tumor invasiveness while impairing immune cell infiltration. Additionally, temozolomide treatment reduces immune cell motility but enhances anti-tumor immune responses. These insights highlight the critical roles of extracellular matrix mechanics and chemotherapy in tumor progression and immune modulation, establishing this device as a powerful tool for studying glioblastoma-immune dynamics and evaluating therapeutic strategies. 000150504 536__ $$9info:eu-repo/grantAgreement/ES/AEI/PID2021-126051OB-C41$$9info:eu-repo/grantAgreement/ES/DGA/T62-230R$$9info:eu-repo/grantAgreement/ES/ISCIII/PT23-00146$$9info:eu-repo/grantAgreement/ES/MINECO/DIN2020-011544 000150504 540__ $$9info:eu-repo/semantics/closedAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/ 000150504 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000150504 700__ $$aOlaizola-Rodrigo, Claudia 000150504 700__ $$aSharko, Vira 000150504 700__ $$aAshrafi, Mehran 000150504 700__ $$0(orcid)0000-0002-5380-6863$$aBarrio, Jesús del$$uUniversidad de Zaragoza 000150504 700__ $$0(orcid)0000-0001-8741-6452$$aDoblaré, Manuel$$uUniversidad de Zaragoza 000150504 700__ $$0(orcid)0000-0003-0734-129X$$aMonge, Rosa 000150504 700__ $$0(orcid)0000-0003-2410-5678$$aOchoa, Ignacio$$uUniversidad de Zaragoza 000150504 700__ $$0(orcid)0000-0003-0156-4230$$aOliván, Sara$$uUniversidad de Zaragoza 000150504 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Histología 000150504 7102_ $$12013$$2765$$aUniversidad de Zaragoza$$bDpto. Química Orgánica$$cÁrea Química Orgánica 000150504 7102_ $$15004$$2605$$aUniversidad de Zaragoza$$bDpto. Ingeniería Mecánica$$cÁrea Mec.Med.Cont. y Teor.Est. 000150504 773__ $$g21, 9 (2025), e2409229 [14 pp.]$$pSmall$$tSmall$$x1613-6810 000150504 8564_ $$s4017837$$uhttps://zaguan.unizar.es/record/150504/files/texto_completo.pdf$$yVersión publicada 000150504 8564_ $$s2507280$$uhttps://zaguan.unizar.es/record/150504/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000150504 909CO $$ooai:zaguan.unizar.es:150504$$particulos$$pdriver 000150504 951__ $$a2025-04-11-15:05:16 000150504 980__ $$aARTICLE