000150520 001__ 150520 000150520 005__ 20251017144641.0 000150520 0247_ $$2doi$$a10.1016/bs.mcb.2024.05.010 000150520 0248_ $$2sideral$$a142582 000150520 037__ $$aART-2024-142582 000150520 041__ $$aeng 000150520 100__ $$aSoler-Agesta, Ruth$$uUniversidad de Zaragoza 000150520 245__ $$aGeneration of transmitochondrial cybrids in cancer cells 000150520 260__ $$c2024 000150520 5060_ $$aAccess copy available to the general public$$fUnrestricted 000150520 5203_ $$aAt odds with historical views suggesting that mitochondrial functions are largely dispensable for cancer cells, it is now clear that mitochondria have a major impact on malignant transformation, tumor progression and response to treatment. Mitochondria are indeed critical for neoplastic cells not only as an abundant source of ATP and other metabolic intermediates, but also as gatekeepers of apoptotic cell death and inflammation. Interestingly, while mitochondrial components are mostly encoded by nuclear genes, mitochondria contain a small, circular genome that codes for a few mitochondrial proteins, ribosomal RNAs and transfer RNAs. Here, we describe a straightforward method to generate transmitochondrial cybrids, i.e., cancer cells depleted of their mitochondrial DNA and reconstituted with intact mitochondria from another cellular source. Once established, transmitochondrial cybrids can be stably propagated and are valuable to dissect the specific impact of the mitochondrial genome on cancer cell functions. 000150520 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/ 000150520 592__ $$a0.296$$b2024 000150520 593__ $$aCell Biology$$c2024$$dQ4 000150520 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion 000150520 700__ $$aRipollés-Yuba, Cristina$$uUniversidad de Zaragoza 000150520 700__ $$aMarco-Brualla, Joaquín 000150520 700__ $$0(orcid)0000-0002-6600-1618$$aMoreno-Loshuertos, Raquel$$uUniversidad de Zaragoza 000150520 700__ $$aSato, Ai 000150520 700__ $$aBeltrán-Visiedo, Manuel 000150520 700__ $$aGalluzzi, Lorenzo 000150520 700__ $$0(orcid)0000-0002-5175-8394$$aAnel, Alberto$$uUniversidad de Zaragoza 000150520 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole. 000150520 773__ $$g189 (2024), 23-40$$pMethods cell biol.$$tMETHODS IN CELL BIOLOGY$$x0091-679X 000150520 8564_ $$s829678$$uhttps://zaguan.unizar.es/record/150520/files/texto_completo.pdf$$yPostprint 000150520 8564_ $$s697071$$uhttps://zaguan.unizar.es/record/150520/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint 000150520 909CO $$ooai:zaguan.unizar.es:150520$$particulos$$pdriver 000150520 951__ $$a2025-10-17-14:32:23 000150520 980__ $$aARTICLE