150524 20260113074939.0 doi 10.1016/j.ijpharm.2025.125196 sideral 142584 ART-2025-142584 eng Santos-Vizcaino, Edorta Genipin-crosslinked double PLL membranes overcome the strength-diffusion trade-off in cell encapsulation without compromising biocompatibility 2025 Access copy available to the general public Unrestricted Cell microencapsulation technologies allow non-autologous implantation of therapeutic cells for sustained drug delivery purposes. The perm-selective membrane of these systems provides resistance to rupture, stablishes the upper molecular weight limit in bidirectional diffusion of molecules, and affects biocompatibility. Thus, despite being a decisive factor to succeed in terms of biosafety and therapeutic efficacy, little progress has been made in its optimization so far. Here we show that, compared to other usually used coating designs, genipin-crosslinked double poly-L-lysine (GDP) membranes are able to simultaneously improve mechanical and mass-transport properties of the microcapsules, without causing any significant increase in the foreign body response when implanted in vivo. In particular, we show that GDP membranes confer capsular integrity under high pressures, both internal and external. Furthermore, this membrane design allows for more efficient bidirectional diffusion of molecules in the 20–40 kDa range while preserving the molecular weight cut-off required for exerting an effective immunobarrier. These findings may also be useful for optimizing the membrane characteristics of multiple drug delivery systems. info:eu-repo/semantics/openAccess by-nc-nd https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Virumbrales-Muñoz, María Gonzalez-Pujana, Ainhoa Luker, Gary D. (orcid)0000-0003-2410-5678 Ochoa, Ignacio Universidad de Zaragoza Hernandez, Rosa Maria Pedraz, Jose Luis 1003 443 Universidad de Zaragoza Dpto. Anatom.Histolog.Humanas Area Histología 670 (2025), 125196 [11 pp.] Int. j. pharm. International Journal of Pharmaceutics 0378-5173 1044836 http://zaguan.unizar.es/record/150524/files/texto_completo.pdf Postprint info:eu-repo/date/embargoEnd/2026-01-10 1360752 http://zaguan.unizar.es/record/150524/files/texto_completo.jpg?subformat=icon icon Postprint info:eu-repo/date/embargoEnd/2026-01-10 oai:zaguan.unizar.es:150524 articulos driver 2026-01-12-13:01:41 ARTICLE