000150552 001__ 150552
000150552 005__ 20250210102457.0
000150552 0247_ $$2doi$$a10.1016/j.rvsc.2024.105488
000150552 0248_ $$2sideral$$a142625
000150552 037__ $$aART-2024-142625
000150552 041__ $$aeng
000150552 100__ $$0(orcid)0000-0003-4970-2460$$aLacueva-Aparicio, A.
000150552 245__ $$aPreliminary steps for fabrication of microfluidic systems for swine sperm sorting: Materials, perfusing systems and flow
000150552 260__ $$c2024
000150552 5060_ $$aAccess copy available to the general public$$fUnrestricted
000150552 5203_ $$aThe success rate of assisted reproductive techniques in the livestock production can be optimized by improving the quality of the semen sample by selecting only the good quality sperm from the ejaculate. Microfluidic technology has been studied for sperm sorting mainly in human ejaculates but has not been studied for boar sperm. Spermatozoa have been proven to be highly sensitive to different microplastics, but the potential toxic effects of the materials used to set up microfluidic systems have not been studied. The main goal of this study was to assess the possible toxic effect on boar sperm of materials commonly used for a microfluidic system and to evaluate the effect of different flow control systems (peristaltic pump, syringe pump and a microfluidic flow controller) at different flow rates (10 μl*min-1, 100 μl*min-1 and 1 ml*min-1) on sperm quality, as preliminary information for the development of a swine sperm sorting microfluidic system. Results showed no negative effect of the different materials at different concentrations. The control reached the highest curvilinear velocity compared to the peristaltic pump and the pressure-based flow control system. In the flow rates, 10 μm*min-1 showed the poorest results and no significant differences were observed between control and 1 mlmin-1 flow in any of the parameters. In conclusion, all materials that were studied for microfluidic fabrications were suitable for sperm sorting, any of the pumps would be suitable for sperm selection and 1 ml*min-1 flow rate would be the flow rate of choice for sperm pumping.
000150552 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000150552 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000150552 700__ $$aMonge, R.
000150552 700__ $$aSerrano, L.
000150552 700__ $$aMalo, C.$$uUniversidad de Zaragoza
000150552 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000150552 773__ $$g183 (2024), 105488 [6 pp.]$$pRes. Vet. Sci.$$tRESEARCH IN VETERINARY SCIENCE$$x0034-5288
000150552 8564_ $$s1823273$$uhttps://zaguan.unizar.es/record/150552/files/texto_completo.pdf$$yVersión publicada
000150552 8564_ $$s2559308$$uhttps://zaguan.unizar.es/record/150552/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000150552 909CO $$ooai:zaguan.unizar.es:150552$$particulos$$pdriver
000150552 951__ $$a2025-02-10-08:28:08
000150552 980__ $$aARTICLE