000150629 001__ 150629 000150629 005__ 20251017144557.0 000150629 0247_ $$2doi$$a10.1016/j.livsci.2020.104071 000150629 0248_ $$2sideral$$a117720 000150629 037__ $$aART-2020-117720 000150629 041__ $$aeng 000150629 100__ $$0(orcid)0000-0003-3927-9793$$aYuste, S. 000150629 245__ $$aEffects of partial substitution of barley with maize and sugar beet pulp on growth performance, rumen fermentation and microbial diversity shift of beef calves during transition from a milk and pasture regimen to a high-concentrate diet 000150629 260__ $$c2020 000150629 5060_ $$aAccess copy available to the general public$$fUnrestricted 000150629 5203_ $$aEffects of partial substitution of barley with maize and beet pulp (BP) on animal performance, rumen fermentation and rumen microbial populations were evaluated in thirty-nine newly weaned calves transitioned from a milk and pasture regime to a high-concentrate ration. Diets were: a barley-based growing diet (C); and two diets in which barley was partially replaced with maize and 10% BP (BP10) or 22% BP (BP22) on as fed basis. Wheat straw was offered ad libitum in all cases. Three adaptation protocols were followed: in Protocol 1 animals were abruptly shifted to the C diet, whereas calves in Protocols 2 and 3 were gradually adapted and received diets BP10 and BP22, respectively, for 10d; thereafter a 50:50 mixture of their diet and diet C until day 14, and finally, from day 15, diet C. All animals were slaughtered at a target live weight (LW) of ca. 500 kg. The experiment had then three phases (Ph): Ph1 (0-10d), Ph2 (11-14d) and Ph3 (15d-slaughter). Concentrate intake and proportion of straw in the diet were affected (P<0.01) by the interaction dietary protocol by phase, showing animals in Protocol 3 the lowest concentrate intake and the highest straw proportion in Ph2. Daily gains, LW at the end of each phase, dressing percentage (59 ± 0.4%, on average) and total concentrate intake along the experiment (995 ± 21.3 kg DM, on average) were similar (P>0.05) among adaptation protocols. Daily rumen pH, and concentrations of lactate and volatile fatty acids were not affected by protocol. At the end of the trial, protozoa persisted in the rumen of all calves with an average concentration of 5.89 log cells/mL. Diversity dropped throughout the study; most protozoa belonged to genera Entodinium (97.5%) and Isotricha (1.7%), with minor presence of Dasytricha (0.05%), Epidinium (0.43%) and Subfamily Diplodiniinae (0.23%). Adaptation protocol had no effect on rumen bacterial population or diversity; however, both were affected by day of sampling indicating a shift in bacterial community in response to the concentrate inclusion. Although diversity dropped with concentrate inclusion, there was an increase after d10. In conclusion, the application of different protocols to adapt beef cattle to high-concentrate diets had no effect on animal performance and rumen fermentation which were not negatively affected by the abrupt transition to the high-concentrate diet. This suggests that calves might cope with those feeding conditions probably due to the microbial adaptions to a new rumen environment. 000150629 536__ $$9info:eu-repo/grantAgreement/ES/MINECO-DGA-FSE/AGL2013-46820 000150629 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es 000150629 590__ $$a1.943$$b2020 000150629 591__ $$aAGRICULTURE, DAIRY & ANIMAL SCIENCE$$b28 / 63 = 0.444$$c2020$$dQ2$$eT2 000150629 592__ $$a0.622$$b2020 000150629 593__ $$aVeterinary (miscellaneous)$$c2020$$dQ1 000150629 593__ $$aAnimal Science and Zoology$$c2020$$dQ1 000150629 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion 000150629 700__ $$0(orcid)0000-0001-8932-4615$$aAmanzougarene, Z. 000150629 700__ $$ade la Fuente, G. 000150629 700__ $$0(orcid)0000-0002-0712-1185$$aFondevila, M.$$uUniversidad de Zaragoza 000150629 700__ $$0(orcid)0000-0002-8753-8887$$ade Vega, A.$$uUniversidad de Zaragoza 000150629 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal 000150629 773__ $$g238 (2020), 104071 [11 pp.]$$pLivest. Sci.$$tLivestock Science$$x1871-1413 000150629 8564_ $$s344216$$uhttps://zaguan.unizar.es/record/150629/files/texto_completo.pdf$$yPostprint 000150629 8564_ $$s589798$$uhttps://zaguan.unizar.es/record/150629/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint 000150629 909CO $$ooai:zaguan.unizar.es:150629$$particulos$$pdriver 000150629 951__ $$a2025-10-17-14:13:36 000150629 980__ $$aARTICLE