000150713 001__ 150713
000150713 005__ 20251017144642.0
000150713 0247_ $$2doi$$a10.1021/jacs.4c12637
000150713 0248_ $$2sideral$$a142857
000150713 037__ $$aART-2025-142857
000150713 041__ $$aeng
000150713 100__ $$aPostigo, Alejandro
000150713 245__ $$aFolding and Functionalizing DNA Origami: A Versatile Approach Using a Reactive Polyamine
000150713 260__ $$c2025
000150713 5060_ $$aAccess copy available to the general public$$fUnrestricted
000150713 5203_ $$aDNA nanotechnology is a powerful synthetic approach to crafting diverse nanostructures through self-assembly. Chemical decoration of such nanostructures is often required to tailor their properties for specific applications. In this Letter, we introduce a pioneering method to direct the assembly and enable the functionalization of DNA nanostructures using an azide-bearing functional polyamine. We first demonstrate the successful polyamine-assisted folding of a scaffolded DNA origami nanostructure equipped with reactive azide groups. Leveraging this reactivity, we next showcase the decoration of the DNA origami via strain-promoted azide–alkyne cycloaddition with dibenzocyclooctyne-containing functional molecules. Specifically, we incorporate a fluorophore (Cy5), polyethylene glycol (PEG), and a hydrophobic phosphatidylethanolamine (PE) tag to tailor the properties of our DNA origami nanostructures. Our approach is expected to streamline and reduce the cost of chemical customization of intricate DNA nanostructures, paving the way for enhanced versatility and applicability.
000150713 536__ $$9info:eu-repo/grantAgreement/ES/AEI/CEX2023-001286-S$$9info:eu-repo/grantAgreement/ES/CSIC/PTI-001$$9info:eu-repo/grantAgreement/ES/DGA/E09-23R-QMAD$$9info:eu-repo/grantAgreement/ES/DGA/E47-23R$$9info:eu-repo/grantAgreement/ES/MICINN-AEI/PRTR-C17.I1$$9info:eu-repo/grantAgreement/ES/MICINN/CTQ2017-84087-R$$9info:eu-repo/grantAgreement/ES/MCINN/PID2020-113003GB-I00$$9info:eu-repo/grantAgreement/ES/MICINN/PID2023-147656OB-I00$$9info:eu-repo/grantAgreement/ES/MICINN PRE2021-098521$$9info:eu-repo/grantAgreement/ES/MICINN/RYC-2015-18471
000150713 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000150713 655_4 $$ainfo:eu-repo/semantics/conferenceObject$$vinfo:eu-repo/semantics/publishedVersion
000150713 700__ $$0(orcid)0000-0003-3459-8605$$aMarcuello, Carlos
000150713 700__ $$aVerstraeten, William
000150713 700__ $$aSarasa, Santiago$$uUniversidad de Zaragoza
000150713 700__ $$aWalther, Tobias
000150713 700__ $$0(orcid)0000-0001-7460-5916$$aLostao, Anabel
000150713 700__ $$aGöpfrich, Kerstin
000150713 700__ $$0(orcid)0000-0002-5380-6863$$aBarrio, Jesús del$$uUniversidad de Zaragoza
000150713 700__ $$0(orcid)0000-0003-3109-4284$$aHernández-Ainsa, Silvia
000150713 7102_ $$12013$$2765$$aUniversidad de Zaragoza$$bDpto. Química Orgánica$$cÁrea Química Orgánica
000150713 773__ $$g147, 5 (2025), 3919-3924$$pJ. Am. Chem. Soc.$$tJournal of the American Chemical Society$$x0002-7863
000150713 8564_ $$s4325652$$uhttps://zaguan.unizar.es/record/150713/files/texto_completo.pdf$$yVersión publicada
000150713 8564_ $$s3344317$$uhttps://zaguan.unizar.es/record/150713/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000150713 909CO $$ooai:zaguan.unizar.es:150713$$particulos$$pdriver
000150713 951__ $$a2025-10-17-14:32:28
000150713 980__ $$aARTICLE