150713 20251017144642.0 doi 10.1021/jacs.4c12637 sideral 142857 ART-2025-142857 eng Postigo, Alejandro Folding and Functionalizing DNA Origami: A Versatile Approach Using a Reactive Polyamine 2025 Access copy available to the general public Unrestricted DNA nanotechnology is a powerful synthetic approach to crafting diverse nanostructures through self-assembly. Chemical decoration of such nanostructures is often required to tailor their properties for specific applications. In this Letter, we introduce a pioneering method to direct the assembly and enable the functionalization of DNA nanostructures using an azide-bearing functional polyamine. We first demonstrate the successful polyamine-assisted folding of a scaffolded DNA origami nanostructure equipped with reactive azide groups. Leveraging this reactivity, we next showcase the decoration of the DNA origami via strain-promoted azide–alkyne cycloaddition with dibenzocyclooctyne-containing functional molecules. Specifically, we incorporate a fluorophore (Cy5), polyethylene glycol (PEG), and a hydrophobic phosphatidylethanolamine (PE) tag to tailor the properties of our DNA origami nanostructures. Our approach is expected to streamline and reduce the cost of chemical customization of intricate DNA nanostructures, paving the way for enhanced versatility and applicability. info:eu-repo/grantAgreement/ES/AEI/CEX2023-001286-S info:eu-repo/grantAgreement/ES/CSIC/PTI-001 info:eu-repo/grantAgreement/ES/DGA/E09-23R-QMAD info:eu-repo/grantAgreement/ES/DGA/E47-23R info:eu-repo/grantAgreement/ES/MICINN-AEI/PRTR-C17.I1 info:eu-repo/grantAgreement/ES/MICINN/CTQ2017-84087-R info:eu-repo/grantAgreement/ES/MCINN/PID2020-113003GB-I00 info:eu-repo/grantAgreement/ES/MICINN/PID2023-147656OB-I00 info:eu-repo/grantAgreement/ES/MICINN PRE2021-098521 info:eu-repo/grantAgreement/ES/MICINN/RYC-2015-18471 info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es info:eu-repo/semantics/conferenceObject info:eu-repo/semantics/publishedVersion Marcuello, Carlos (orcid)0000-0003-3459-8605 Verstraeten, William Sarasa, Santiago Universidad de Zaragoza Walther, Tobias Lostao, Anabel (orcid)0000-0001-7460-5916 Göpfrich, Kerstin Barrio, Jesús del Universidad de Zaragoza (orcid)0000-0002-5380-6863 Hernández-Ainsa, Silvia (orcid)0000-0003-3109-4284 2013 765 Universidad de Zaragoza Dpto. Química Orgánica Área Química Orgánica 147, 5 (2025), 3919-3924 J. Am. Chem. Soc. Journal of the American Chemical Society 0002-7863 4325652 http://zaguan.unizar.es/record/150713/files/texto_completo.pdf Versión publicada 3344317 http://zaguan.unizar.es/record/150713/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:150713 articulos driver 2025-10-17-14:32:28 ARTICLE