000150749 001__ 150749
000150749 005__ 20250214153851.0
000150749 0247_ $$2doi$$a10.1007/s00604-025-06964-x
000150749 0248_ $$2sideral$$a142806
000150749 037__ $$aART-2025-142806
000150749 041__ $$aeng
000150749 100__ $$aDomínguez, Mario
000150749 245__ $$aNADPH-mediated seedless in situ formation of gold or gold-platinum nanoparticles for the enzymatic determination of atropine
000150749 260__ $$c2025
000150749 5060_ $$aAccess copy available to the general public$$fUnrestricted
000150749 5203_ $$aThe without-seeds in situ formation of gold nanoparticles from NADPH and its application to the colorimetric determination of atropine (a tropane alkaloid) in cereals is reported. The method is based on a chemical hydrolysis, followed by an enzymatic oxidation by NADP catalyzed by tropinone reductase in the presence of Au(III) or Au(III)/Pt(II). During this reaction, the formed NADPH reduces the metal ion precursor to AuNPs (or AuPtNPs) and the absorption due to the plasmon band (550 nm or 575 nm) is measured. The method (AuPtNPs) allows the determination of the analyte in the concentration range 0.025 to 0.09 mM with an RSD of 3% (n = 5) and is applied to its determination in spiked buckwheat samples using the standard addition method, with 96.9 ± 2.0% recovery. It is also demonstrated that NAD(P)H and NADH have different kinetics for AuNP generation, which could be used to discriminate between these two cofactors.
000150749 536__ $$9info:eu-repo/grantAgreement/ES/DGA/E25-23R$$9info:eu-repo/grantAgreement/ES/MICINN/PID2019-105408GB-I00$$9info:eu-repo/grantAgreement/ES/MICINN/PID2022-139235OB-I00
000150749 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000150749 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000150749 700__ $$0(orcid)0000-0002-7902-6005$$aMarcos, Susana de$$uUniversidad de Zaragoza
000150749 700__ $$0(orcid)0000-0002-8973-5104$$aGalbán, Javier$$uUniversidad de Zaragoza
000150749 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica
000150749 773__ $$g192, 3 (2025), 132 [10 pp.]$$pMikrochim. acta$$tMicrochimica Acta$$x0026-3672
000150749 8564_ $$s1346178$$uhttps://zaguan.unizar.es/record/150749/files/texto_completo.pdf$$yVersión publicada
000150749 8564_ $$s2585963$$uhttps://zaguan.unizar.es/record/150749/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000150749 909CO $$ooai:zaguan.unizar.es:150749$$particulos$$pdriver
000150749 951__ $$a2025-02-14-14:03:55
000150749 980__ $$aARTICLE