150749 20251017144649.0 doi 10.1007/s00604-025-06964-x sideral 142806 ART-2025-142806 eng Domínguez, Mario NADPH-mediated seedless in situ formation of gold or gold-platinum nanoparticles for the enzymatic determination of atropine 2025 Access copy available to the general public Unrestricted The without-seeds in situ formation of gold nanoparticles from NADPH and its application to the colorimetric determination of atropine (a tropane alkaloid) in cereals is reported. The method is based on a chemical hydrolysis, followed by an enzymatic oxidation by NADP catalyzed by tropinone reductase in the presence of Au(III) or Au(III)/Pt(II). During this reaction, the formed NADPH reduces the metal ion precursor to AuNPs (or AuPtNPs) and the absorption due to the plasmon band (550 nm or 575 nm) is measured. The method (AuPtNPs) allows the determination of the analyte in the concentration range 0.025 to 0.09 mM with an RSD of 3% (n = 5) and is applied to its determination in spiked buckwheat samples using the standard addition method, with 96.9 ± 2.0% recovery. It is also demonstrated that NAD(P)H and NADH have different kinetics for AuNP generation, which could be used to discriminate between these two cofactors. info:eu-repo/grantAgreement/ES/DGA/E25-23R info:eu-repo/grantAgreement/ES/MICINN/PID2019-105408GB-I00 info:eu-repo/grantAgreement/ES/MICINN/PID2022-139235OB-I00 info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Marcos, Susana de Universidad de Zaragoza (orcid)0000-0002-7902-6005 Galbán, Javier Universidad de Zaragoza (orcid)0000-0002-8973-5104 2009 750 Universidad de Zaragoza Dpto. Química Analítica Área Química Analítica 192, 3 (2025), 132 [10 pp.] Mikrochim. acta Microchimica Acta 0026-3672 1346178 http://zaguan.unizar.es/record/150749/files/texto_completo.pdf Versión publicada 2585963 http://zaguan.unizar.es/record/150749/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:150749 articulos driver 2025-10-17-14:35:34 ARTICLE