000151153 001__ 151153
000151153 005__ 20251017144608.0
000151153 0247_ $$2doi$$a10.1021/acsami.1c02338
000151153 0248_ $$2sideral$$a124397
000151153 037__ $$aART-2021-124397
000151153 041__ $$aeng
000151153 100__ $$0(orcid)0000-0003-4516-8694$$aBeola, L.
000151153 245__ $$aCritical Parameters to Improve Pancreatic Cancer Treatment Using Magnetic Hyperthermia: Field Conditions, Immune Response, and Particle Biodistribution
000151153 260__ $$c2021
000151153 5060_ $$aAccess copy available to the general public$$fUnrestricted
000151153 5203_ $$aMagnetic hyperthermia (MH) was used to treat a murine model of pancreatic cancer. This type of cancer is generally characterized by the presence of dense stroma that acts as a barrier for chemotherapeutic treatments. Several alternating magnetic field (AMF) conditions were evaluated using three-dimensional (3D) cell culture models loaded with magnetic nanoparticles (MNPs) to determine which conditions were producing a strong effect on the cell viability. Once the optimal AMF conditions were selected, in vivo experiments were carried out using similar frequency and field amplitude parameters. A marker of the immune response activation, calreticulin (CALR), was evaluated in cells from a xenograft tumor model after the MH treatment. Moreover, the distribution of nanoparticles within the tumor tissue was assessed by histological analysis of tumor sections, observing that the exposure to the alternating magnetic field resulted in the migration of particles toward the inner parts of the tumor. Finally, a relationship between an inadequate body biodistribution of the particles after their intratumoral injection and a significant decrease in the effectiveness of the MH treatment was found. Animals in which most of the particles remained in the tumor area after injection showed higher reductions in the tumor volume growth in comparison with those animals in which part of the particles were found also in the liver and spleen. Therefore, our results point out several factors that should be considered to improve the treatment effectiveness of pancreatic cancer by magnetic hyperthermia.
000151153 536__ $$9info:eu-repo/grantAgreement/ES/MCIU-AEI-FEDER/BIO2017-84246-C2-1-R$$9info:eu-repo/grantAgreement/EUR/M-ERA.NET/COFUND-MagicCellGene-PCIN-2017-060$$9info:eu-repo/grantAgreement/ES/MINECO/PGC2018-096016-B-I00
000151153 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000151153 590__ $$a10.383$$b2021
000151153 591__ $$aNANOSCIENCE & NANOTECHNOLOGY$$b23 / 108 = 0.213$$c2021$$dQ1$$eT1
000151153 591__ $$aMATERIALS SCIENCE, MULTIDISCIPLINARY$$b49 / 344 = 0.142$$c2021$$dQ1$$eT1
000151153 592__ $$a2.143$$b2021
000151153 593__ $$aMedicine (miscellaneous)$$c2021$$dQ1
000151153 593__ $$aMaterials Science (miscellaneous)$$c2021$$dQ1
000151153 594__ $$a14.4$$b2021
000151153 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000151153 700__ $$0(orcid)0000-0001-6170-4237$$aGrazu, V.
000151153 700__ $$0(orcid)0000-0002-0970-1917$$aFernandez-Afonso, Y.
000151153 700__ $$0(orcid)0000-0001-5559-8757$$aFratila, R.M.
000151153 700__ $$0(orcid)0000-0003-3792-287X$$aDe Las Heras, M.$$uUniversidad de Zaragoza
000151153 700__ $$aDe La Fuente, J.M.
000151153 700__ $$0(orcid)0000-0003-2366-3598$$aGutierrez, L.$$uUniversidad de Zaragoza
000151153 700__ $$aAsin, L.
000151153 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica
000151153 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000151153 773__ $$g13, 11 (2021), 12982–12996$$pACS appl. mater. interfaces$$tACS applied materials & interfaces$$x1944-8244
000151153 8564_ $$s6594092$$uhttps://zaguan.unizar.es/record/151153/files/texto_completo.pdf$$yVersión publicada
000151153 8564_ $$s3261109$$uhttps://zaguan.unizar.es/record/151153/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000151153 909CO $$ooai:zaguan.unizar.es:151153$$particulos$$pdriver
000151153 951__ $$a2025-10-17-14:16:11
000151153 980__ $$aARTICLE