000151156 001__ 151156
000151156 005__ 20250227101504.0
000151156 0247_ $$2doi$$a10.1016/j.talanta.2021.122644
000151156 0248_ $$2sideral$$a125787
000151156 037__ $$aART-2021-125787
000151156 041__ $$aeng
000151156 100__ $$0(orcid)0000-0003-1599-8216$$aLanda G.$$uUniversidad de Zaragoza
000151156 245__ $$aSelective point-of-care detection of pathogenic bacteria using sialic acid functionalized gold nanoparticles
000151156 260__ $$c2021
000151156 5060_ $$aAccess copy available to the general public$$fUnrestricted
000151156 5203_ $$aIn resource-limited settings, fast and simple point-of-need tests should facilitate healthcare providers the identification of pathogens avoiding empirical suboptimal treatments with broad-spectrum antibiotics. A rapid optical whole cell bacterial biosensor has been here developed using sialic acid functionalized gold nanoparticles allowing the selective screening of Gram-positive Staphylococcus aureus ATCC 25923 and Methicillin Resistant Staphylococcus aureus (MRSA) USA300 and Gram-negative bacteria (Pseudomonas aeruginosa ATCC 15442) by selecting the appropriate dispersing media. Those bacteria were selected due to their common presence in wound bed tissue of chronic infected topical wounds. The discrimination of bacterial pathogens has been attempted in different media including water, two independent buffers, bacterial broth, human serum and human urine. The identification of Gram + bacterial pathogens was also assessed under simultaneous co-culture of S. Aureus and Pseudomonas aeruginosa. High bacterial loads were required to provide with a statistically significant optical pathogen identification in human serum whereas it was not possible to detect the presence of bacteria at clinically relevant levels in urine. © 2021 The Authors
000151156 536__ $$9info:eu-repo/grantAgreement/EUR/ERC-2013-CoG-614715-NANOHEDONISM$$9info:eu-repo/grantAgreement/ES/MCINN/PRE2018-085769$$9info:eu-repo/grantAgreement/ES/MICINN/RTI2018-099019-A-I00$$9info:eu-repo/grantAgreement/ES/MINECO/CTQ2014-52384-R
000151156 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000151156 590__ $$a6.556$$b2021
000151156 591__ $$aCHEMISTRY, ANALYTICAL$$b11 / 87 = 0.126$$c2021$$dQ1$$eT1
000151156 592__ $$a0.983$$b2021
000151156 593__ $$aSpectroscopy$$c2021$$dQ1
000151156 593__ $$aAnalytical Chemistry$$c2021$$dQ1
000151156 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000151156 700__ $$aMiranda-Calderon L.G.
000151156 700__ $$0(orcid)0000-0002-6873-5244$$aSebastian V.$$uUniversidad de Zaragoza
000151156 700__ $$0(orcid)0000-0002-2966-9088$$aIrusta S.$$uUniversidad de Zaragoza
000151156 700__ $$aMendoza G.
000151156 700__ $$0(orcid)0000-0003-3165-0156$$aArruebo M.$$uUniversidad de Zaragoza
000151156 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química
000151156 7102_ $$15005$$2790$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Tecnologi. Medio Ambiente
000151156 773__ $$g234 (2021), 122644 [10 pp.]$$pTalanta$$tTalanta$$x0039-9140
000151156 8564_ $$s7303864$$uhttps://zaguan.unizar.es/record/151156/files/texto_completo.pdf$$yVersión publicada
000151156 8564_ $$s2594481$$uhttps://zaguan.unizar.es/record/151156/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000151156 909CO $$ooai:zaguan.unizar.es:151156$$particulos$$pdriver
000151156 951__ $$a2025-02-27-09:26:30
000151156 980__ $$aARTICLE