151156 20251017144608.0 doi 10.1016/j.talanta.2021.122644 sideral 125787 ART-2021-125787 eng Landa G. Universidad de Zaragoza (orcid)0000-0003-1599-8216 Selective point-of-care detection of pathogenic bacteria using sialic acid functionalized gold nanoparticles 2021 Access copy available to the general public Unrestricted In resource-limited settings, fast and simple point-of-need tests should facilitate healthcare providers the identification of pathogens avoiding empirical suboptimal treatments with broad-spectrum antibiotics. A rapid optical whole cell bacterial biosensor has been here developed using sialic acid functionalized gold nanoparticles allowing the selective screening of Gram-positive Staphylococcus aureus ATCC 25923 and Methicillin Resistant Staphylococcus aureus (MRSA) USA300 and Gram-negative bacteria (Pseudomonas aeruginosa ATCC 15442) by selecting the appropriate dispersing media. Those bacteria were selected due to their common presence in wound bed tissue of chronic infected topical wounds. The discrimination of bacterial pathogens has been attempted in different media including water, two independent buffers, bacterial broth, human serum and human urine. The identification of Gram + bacterial pathogens was also assessed under simultaneous co-culture of S. Aureus and Pseudomonas aeruginosa. High bacterial loads were required to provide with a statistically significant optical pathogen identification in human serum whereas it was not possible to detect the presence of bacteria at clinically relevant levels in urine. © 2021 The Authors info:eu-repo/grantAgreement/EUR/ERC-2013-CoG-614715-NANOHEDONISM info:eu-repo/grantAgreement/ES/MCINN/PRE2018-085769 info:eu-repo/grantAgreement/ES/MICINN/RTI2018-099019-A-I00 info:eu-repo/grantAgreement/ES/MINECO/CTQ2014-52384-R info:eu-repo/semantics/openAccess by-nc-nd https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es 6.556 2021 CHEMISTRY, ANALYTICAL 11 / 87 = 0.126 2021 Q1 T1 0.983 2021 Spectroscopy 2021 Q1 Analytical Chemistry 2021 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Miranda-Calderon L.G. Sebastian V. Universidad de Zaragoza (orcid)0000-0002-6873-5244 Irusta S. Universidad de Zaragoza (orcid)0000-0002-2966-9088 Mendoza G. Arruebo M. Universidad de Zaragoza (orcid)0000-0003-3165-0156 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 5005 790 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Tecnologi. Medio Ambiente 234 (2021), 122644 [10 pp.] Talanta Talanta 0039-9140 7303864 http://zaguan.unizar.es/record/151156/files/texto_completo.pdf Versión publicada 2594481 http://zaguan.unizar.es/record/151156/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:151156 articulos driver 2025-10-17-14:16:18 ARTICLE