151171 20251017144609.0 doi 10.3390/ph14080759 sideral 125277 ART-2021-125277 eng Lopez-Tejedor, David In Vitro Antiviral Activity of Tyrosinase from Mushroom Agaricus bisporus against Hepatitis C Virus 2021 Access copy available to the general public Unrestricted Tyrosinases from a commercial Agaricus bisporus protein extract and directly isolated from white mushrooms were purified in order to obtaining the well-known tyrosinase from A. bisporus (TyrAB) of 45 kDa and a newly discovered 50 kDa tyrosinase isoform (Tyr50 kDa), and tested showing high antiviral activity against the hepatitis C virus for the first time. Cell toxicity and antiviral activity of tyrosinases were determined in cultured Huh 5-2 liver tumor cells transfected with a replicon system (a plasmid that includes all non-structural hepatitis C virus proteins and replicates autonomously). TyrAB was able to inhibit the replication of the hepatitis C virus without inducing toxicity in liver cells. In addition, the post-translational isoform Tyr50 kDa showed higher antiviral capacity than the former (up to 10 times greater), also exhibiting 10 times higher activity than the commercial drug Ribavirin\u00ae. This antiviral activity was directly proportional to the enzymatic activity of tyrosinases, as no antiviral capacity was observed in the inactive form of the enzymes. The tyrosinases approach could represent a new antiviral inhibition mechanism, through a plausible catalytic mechanism of selective hydroxylation of the key role of tyrosine residues in viral proteases. info:eu-repo/grantAgreement/ES/DGA/B25-17R info:eu-repo/grantAgreement/ES/DGA/E45-17R info:eu-repo/grantAgreement/ES/ISCIII/CPII13-00017 info:eu-repo/grantAgreement/ES/ISCIII-ERDF-ESF/PI15-00663-Investing in your future info:eu-repo/grantAgreement/ES/ISCIII-ERDF-ESF/PI18-00349-Investing in your future info:eu-repo/grantAgreement/ES/MCIU-AEI-FEDER/BFU2013-47064-P info:eu-repo/grantAgreement/ES/MCIU-AEI-FEDER/BFU2016-78232-P info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es 5.215 2021 PHARMACOLOGY & PHARMACY 69 / 279 = 0.247 2021 Q1 T1 CHEMISTRY, MEDICINAL 16 / 63 = 0.254 2021 Q2 T1 0.851 2021 Pharmaceutical Science 2021 Q1 Drug Discovery 2021 Q1 4.0 2021 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Claveria-Gimeno, Rafael Velazquez-Campoy, Adrian Universidad de Zaragoza (orcid)0000-0001-5702-4538 Abian, Olga Universidad de Zaragoza (orcid)0000-0001-5664-1729 Palomo, Jose M. 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 14, 8 (2021), 759 [12 pp.] Pharmaceuticals Pharmaceuticals 1424-8247 3471209 http://zaguan.unizar.es/record/151171/files/texto_completo.pdf Versión publicada 2441667 http://zaguan.unizar.es/record/151171/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:151171 articulos driver 2025-10-17-14:16:38 ARTICLE