151172 20251017144609.0 doi 10.3390/ijms22126501 sideral 125278 ART-2021-125278 eng Morcuende-Ventura, Violeta Fluorescence Liquid Biopsy for Cancer Detection Is Improved by Using Cationic Dendronized Hyperbranched Polymer 2021 Access copy available to the general public Unrestricted (1) Background: Biophysical techniques applied to serum samples characterization could promote the development of new diagnostic tools. Fluorescence spectroscopy has been previously applied to biological samples from cancer patients and differences from healthy individuals were observed. Dendronized hyperbranched polymers (DHP) based on bis(hydroxymethyl)propionic acid (bis-MPA) were developed in our group and their potential biomedical applications explored. (2) Methods: A total of 94 serum samples from diagnosed cancer patients and healthy individuals were studied (20 pancreatic ductal adenocarcinoma, 25 blood donor, 24 ovarian cancer, and 25 benign ovarian cyst samples). (3) Results: Fluorescence spectra of serum samples (fluorescence liquid biopsy, FLB) in the presence and the absence of DHP-bMPA were recorded and two parameters from the signal curves obtained. A secondary parameter, the fluorescence spectrum score (FSscore), was calculated, and the diagnostic model assessed. For pancreatic ductal adenocarcinoma (PDAC) and ovarian cancer, the classification performance was improved when including DHP-bMPA, achieving high values of statistical sensitivity and specificity (over 85% for both pathologies). (4) Conclusions: We have applied FLB as a quick, simple, and minimally invasive promising technique in cancer diagnosis. The classification performance of the diagnostic method was further improved by using DHP-bMPA, which interacted differentially with serum samples from healthy and diseased subjects. These preliminary results set the basis for a larger study and move FLB closer to its clinical application, providing useful information for the oncologist during patient diagnosis. info:eu-repo/grantAgreement/ES/DGA/B25-17R info:eu-repo/grantAgreement/ES/DGA/E45-17R info:eu-repo/grantAgreement/ES/DGA/E47-20R info:eu-repo/grantAgreement/ES/ISCIII-ERDF-ESF/PI15-00663-Investing in your future info:eu-repo/grantAgreement/ES/ISCIII-ERDF-ESF/PI18-00349-Investing in your future info:eu-repo/grantAgreement/ES/ISCIII-FIS/FI19-00146 info:eu-repo/grantAgreement/ES/MCIU-AEI-FEDER/BFU2016-78232-P info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es 6.208 2021 BIOCHEMISTRY & MOLECULAR BIOLOGY 69 / 297 = 0.232 2021 Q1 T1 CHEMISTRY, MULTIDISCIPLINARY 50 / 179 = 0.279 2021 Q2 T1 1.176 2021 Computer Science Applications 2021 Q1 Inorganic Chemistry 2021 Q1 Spectroscopy 2021 Q1 Organic Chemistry 2021 Q1 Physical and Theoretical Chemistry 2021 Q1 Molecular Biology 2021 Q1 6.9 2021 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Hermoso-Durán, Sonia Abian-Franco, Natalia Universidad de Zaragoza (orcid)0000-0001-5664-1729 Pazo-Cid, Roberto Universidad de Zaragoza (orcid)0000-0002-8026-7391 Ojeda, Jorge L. Universidad de Zaragoza (orcid)0000-0003-0853-2880 Vega, Sonia (orcid)0000-0002-1232-6310 Sanchez-Gracia, Oscar Velazquez-Campoy, Adrian Universidad de Zaragoza (orcid)0000-0001-5702-4538 Sierra, Teresa (orcid)0000-0001-7091-077X Abian, Olga 2007 265 Universidad de Zaragoza Dpto. Métodos Estadísticos Área Estadís. Investig. Opera. 1007 610 Universidad de Zaragoza Dpto. Medicina, Psiqu. y Derm. Area Medicina 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 22, 12 (2021), 6501 [22 pp.] Int. j. mol. sci. International Journal of Molecular Sciences 1661-6596 541112 http://zaguan.unizar.es/record/151172/files/texto_completo.pdf Versión publicada 2729619 http://zaguan.unizar.es/record/151172/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:151172 articulos driver 2025-10-17-14:16:39 ARTICLE