000151351 001__ 151351
000151351 005__ 20250307114715.0
000151351 0247_ $$2doi$$a10.1080/10717544.2021.1998245
000151351 0248_ $$2sideral$$a127147
000151351 037__ $$aART-2021-127147
000151351 041__ $$aeng
000151351 100__ $$aRodrigo M.J.
000151351 245__ $$aLong-term corticosteroid-induced chronic glaucoma model produced by intracameral injection of dexamethasone-loaded PLGA microspheres
000151351 260__ $$c2021
000151351 5060_ $$aAccess copy available to the general public$$fUnrestricted
000151351 5203_ $$aPurpose: To evaluate a new chronic glaucoma model produced by intracameral injection of dexamethasone-loaded poly lactic-co-glycolic acid microspheres (Dex-PLGA-Ms) over six months. Methods: Healthy rats received two injections (at baseline and Week 4) of Dex-PLGA-Ms into the anterior chamber of the right eye. Clinical signs and intraocular pressure (IOP) were weekly recorded. The structure of the retina and optic nerve was in vivo evaluated using optical coherence tomography (OCT) every two weeks and functionally using dark- and light-adapted electroretinography at 0–12–24 weeks. Histological studies were also performed. Results: IOP progressively increased up to hypertension (23.22 ± 3.63 mmHg) in both eyes but did so later in left eyes. OCT quantified a decrease in full-thickness retina posterior pole (R), retinal-nerve-fiber layer (RNFL), and ganglion-cell layer (GCL) thickness up to 24 weeks. Right eyes showed higher neuroretinal thickness loss up to week 8. RNFL experienced the highest percentage thickness loss at the inferior-superior axis, while in GCL the inner sectors of the horizontal axis (Nasal-Temporal) suffered the greatest decrease in thickness. Retinal ganglion cell, photoreceptor, and intermediate cell functionality decreased over time. Increased deposition of collagen IV was also found in zonular fibers and the ciliary body. Conclusions: This work shows the usefulness of drug delivery systems, not to treat pathology but to induce it. Only two injections of Dex-PLGA-Ms in the anterior chamber of rat eyes were enough to progressively create ocular hypertension and subsequent functional and structural neuroretinal degeneration, at least over 6 months.
000151351 536__ $$9info:eu-repo/grantAgreement/ES/ISCIII/M17-00213$$9info:eu-repo/grantAgreement/ES/ISCIII/PI17-01726$$9info:eu-repo/grantAgreement/ES/ISCIII/PI17-01946$$9info:eu-repo/grantAgreement/ES/ISCIII/PI20-00437$$9info:eu-repo/grantAgreement/ES/MINECO-AEI-FEDER/MAT2017-83858-C2-1$$9info:eu-repo/grantAgreement/ES/MINECO-AEI-FEDER/MAT2017-83858-C2-2$$9info:eu-repo/grantAgreement/ES/MINECO-AEI-FEDER/PID2020-113281RB-C22
000151351 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000151351 590__ $$a6.819$$b2021
000151351 591__ $$aPHARMACOLOGY & PHARMACY$$b34 / 279 = 0.122$$c2021$$dQ1$$eT1
000151351 592__ $$a1.092$$b2021
000151351 593__ $$aPharmaceutical Science$$c2021$$dQ1
000151351 593__ $$aMedicine (miscellaneous)$$c2021$$dQ1
000151351 594__ $$a10.2$$b2021
000151351 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000151351 700__ $$aGarcia-Herranz D.
000151351 700__ $$aAragón-Navas A.
000151351 700__ $$aSubias M.
000151351 700__ $$aMartinez-Rincón T.
000151351 700__ $$0(orcid)0000-0001-6072-1545$$aMéndez Martínez S.$$uUniversidad de Zaragoza
000151351 700__ $$0(orcid)0000-0002-7914-4685$$aCardiel García, M.J.$$uUniversidad de Zaragoza
000151351 700__ $$aGarcía-Feijoo J.
000151351 700__ $$aRuberte J.
000151351 700__ $$aHerrero-Vanrell R.
000151351 700__ $$0(orcid)0000-0003-2389-8282$$aPablo Júlvez, L.$$uUniversidad de Zaragoza
000151351 700__ $$0(orcid)0000-0001-6258-2489$$aGarcia Martín, E.$$uUniversidad de Zaragoza
000151351 700__ $$aBravo-Osuna I.
000151351 7102_ $$11013$$2646$$aUniversidad de Zaragoza$$bDpto. Cirugía$$cÁrea Oftalmología
000151351 7102_ $$11013$$2020$$aUniversidad de Zaragoza$$bDpto. Cirugía$$cÁrea Anatomía Patológica
000151351 773__ $$g28, 1 (2021), 2427-2446$$pDrug deliv.$$tDrug Delivery$$x1071-7544
000151351 8564_ $$s4168442$$uhttps://zaguan.unizar.es/record/151351/files/texto_completo.pdf$$yVersión publicada
000151351 8564_ $$s1241580$$uhttps://zaguan.unizar.es/record/151351/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000151351 909CO $$ooai:zaguan.unizar.es:151351$$particulos$$pdriver
000151351 951__ $$a2025-03-07-09:32:50
000151351 980__ $$aARTICLE