000151370 001__ 151370
000151370 005__ 20251017144628.0
000151370 0247_ $$2doi$$a10.1080/23144599.2025.2460923
000151370 0248_ $$2sideral$$a143067
000151370 037__ $$aART-2025-143067
000151370 041__ $$aeng
000151370 100__ $$aDel Río, María Cristina
000151370 245__ $$aMolecular analysis of cryptosporidiosis on cattle farms in Gran Canaria, Canary Islands (Spain)
000151370 260__ $$c2025
000151370 5060_ $$aAccess copy available to the general public$$fUnrestricted
000151370 5203_ $$aCryptosporidium spp. infections in calves cause serious economic losses in livestock and pose an important zoonotic risk. The aim of this study was to investigate the prevalence of Cryptosporidium spp. in cattle on the island of Gran Canaria. Faecal samples were collected from calves and adult cattle from a total of 15 farms, and a questionnaire survey was conducted to farmers. The presence of Cryptosporidium spp. oocysts in faeces was determined by microscopy, showing infection rates of 45.9% in calves and 4.1% in adults, with positive correlation with the faecal scores of infected animals (p < 0.0001). Samples were amplified by PCR targeting SSU rRNA, with positivity rates for calves and adults being 51.7% and 31.7%, respectively. The PCR-positive samples were further genotyped and sequenced for the 60 kDa glycoprotein gene (GP60) and the microsatellite TP14. Four Cryptosporidium species were identified (C. parvum, C. ryanae, C. bovis, and C. andersoni), of which C. parvum was the most frequent in calves (45.8%) and adults (29.2%). GP60 sequencing revealed that all C. parvum samples belonged to the IId family, the most frequent subtypes being IIdA22G1 and IIdA23G1. Overall, the results of this study demonstrate a high occurrence of Cryptosporidium spp. in both calves and adult cattle, including the zoonotic IId family of C. parvum. These findings have significant implications for cattle farming and public health. The lack of awareness among farmers regarding cryptosporidiosis highlights the need for caution to prevent epidemiological outbreaks that could impact both human and livestock health.
000151370 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000151370 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000151370 700__ $$aMartín, Sergio
000151370 700__ $$0(orcid)0000-0001-7831-2483$$aQuílez, Joaquín$$uUniversidad de Zaragoza
000151370 700__ $$0(orcid)0000-0002-9243-7503$$aVergara Castiblanco, Claudia$$uUniversidad de Zaragoza
000151370 700__ $$aMolina, José Manuel
000151370 700__ $$aFerrer, Otilia
000151370 700__ $$aConde, Magnolia María
000151370 700__ $$aMolina, José Adrián
000151370 700__ $$aRuiz, Antonio
000151370 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000151370 7102_ $$11012$$2X$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Técnica. Lab. y Talleres
000151370 773__ $$g13, 1 (2025), 1-14$$pInt. j. vet. sci. med.$$tInternational journal of veterinary sciences and medicine$$x2314-4580
000151370 8564_ $$s2812088$$uhttps://zaguan.unizar.es/record/151370/files/texto_completo.pdf$$yVersión publicada
000151370 8564_ $$s1199035$$uhttps://zaguan.unizar.es/record/151370/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000151370 909CO $$ooai:zaguan.unizar.es:151370$$particulos$$pdriver
000151370 951__ $$a2025-10-17-14:24:55
000151370 980__ $$aARTICLE