000151376 001__ 151376
000151376 005__ 20251017144628.0
000151376 0247_ $$2doi$$a10.3390/ani11061828
000151376 0248_ $$2sideral$$a127256
000151376 037__ $$aART-2021-127256
000151376 041__ $$aeng
000151376 100__ $$0(orcid)0000-0003-3929-5064$$aCarvajal-Serna M.$$uUniversidad de Zaragoza
000151376 245__ $$aSperm behavior and response to melatonin under capacitating conditions in three sheep breeds subject to the equatorial photoperiod
000151376 260__ $$c2021
000151376 5060_ $$aAccess copy available to the general public$$fUnrestricted
000151376 5203_ $$aIn this study, we demonstrated that, in seasonal Mediterranean ovine breeds, supplement-ing the TALP medium with cAMP-elevating agents (the cocktail medium) is effective for achieving ram sperm capacitation, and that melatonin is able to regulate this phenomenon. We investigated the behavior under capacitating conditions using the TALP and cocktail mediums, and the response to melatonin, of spermatozoa from three sheep breeds (Colombian Creole, Romney Marsh, and Hampshire) subject to the equatorial photoperiod, during the dry and the rainy seasons. The cocktail medium was able to induce sperm capacitation, assayed by chlortetracycline staining and phos-photyrosine levels, to a greater extent than TALP, without a higher loss of viability (membrane in-tegrity and viable spermatozoa without phosphatidylserine (PS) translocation). The addition of mel-atonin at 100 pM or 1 µM in the cocktail medium partially prevented the decrease in viability without PS translocation and the increase in capacitated spermatozoa from all breeds, with no significant effect on phosphotyrosine levels. Differences between breeds and seasons were evidenced. This study shows that melatonin is able to exert direct effects on spermatozoa in ovine breeds under equatorial photoperiod conditions, as it does in seasonal breeds located in temperate regions.
000151376 536__ $$9info:eu-repo/grantAgreement/ES/CICYT-FEDER/AGL2017-83799-R$$9info:eu-repo/grantAgreement/ES/DGA/A07-20R
000151376 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000151376 590__ $$a3.231$$b2021
000151376 591__ $$aVETERINARY SCIENCES$$b16 / 145 = 0.11$$c2021$$dQ1$$eT1
000151376 591__ $$aAGRICULTURE, DAIRY & ANIMAL SCIENCE$$b13 / 62 = 0.21$$c2021$$dQ1$$eT1
000151376 592__ $$a0.61$$b2021
000151376 593__ $$aVeterinary (miscellaneous)$$c2021$$dQ1
000151376 593__ $$aAnimal Science and Zoology$$c2021$$dQ1
000151376 594__ $$a2.7$$b2021
000151376 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000151376 700__ $$aCardozo-Cerquera J.A.
000151376 700__ $$aGrajales-Lombana H.A.
000151376 700__ $$0(orcid)0000-0003-1997-4262$$aCasao A.$$uUniversidad de Zaragoza
000151376 700__ $$0(orcid)0000-0002-2312-6402$$aPérez-Pe R.$$uUniversidad de Zaragoza
000151376 7102_ $$11002$$2819$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Zoología
000151376 773__ $$g11, 6 (2021), 1828 [16 pp.]$$pAnimals (Basel)$$tAnimals$$x2076-2615
000151376 8564_ $$s1232511$$uhttps://zaguan.unizar.es/record/151376/files/texto_completo.pdf$$yVersión publicada
000151376 8564_ $$s2713873$$uhttps://zaguan.unizar.es/record/151376/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000151376 909CO $$ooai:zaguan.unizar.es:151376$$particulos$$pdriver
000151376 951__ $$a2025-10-17-14:25:05
000151376 980__ $$aARTICLE