151376 20251017144628.0 doi 10.3390/ani11061828 sideral 127256 ART-2021-127256 eng Carvajal-Serna M. Universidad de Zaragoza (orcid)0000-0003-3929-5064 Sperm behavior and response to melatonin under capacitating conditions in three sheep breeds subject to the equatorial photoperiod 2021 Access copy available to the general public Unrestricted In this study, we demonstrated that, in seasonal Mediterranean ovine breeds, supplement-ing the TALP medium with cAMP-elevating agents (the cocktail medium) is effective for achieving ram sperm capacitation, and that melatonin is able to regulate this phenomenon. We investigated the behavior under capacitating conditions using the TALP and cocktail mediums, and the response to melatonin, of spermatozoa from three sheep breeds (Colombian Creole, Romney Marsh, and Hampshire) subject to the equatorial photoperiod, during the dry and the rainy seasons. The cocktail medium was able to induce sperm capacitation, assayed by chlortetracycline staining and phos-photyrosine levels, to a greater extent than TALP, without a higher loss of viability (membrane in-tegrity and viable spermatozoa without phosphatidylserine (PS) translocation). The addition of mel-atonin at 100 pM or 1 µM in the cocktail medium partially prevented the decrease in viability without PS translocation and the increase in capacitated spermatozoa from all breeds, with no significant effect on phosphotyrosine levels. Differences between breeds and seasons were evidenced. This study shows that melatonin is able to exert direct effects on spermatozoa in ovine breeds under equatorial photoperiod conditions, as it does in seasonal breeds located in temperate regions. info:eu-repo/grantAgreement/ES/CICYT-FEDER/AGL2017-83799-R info:eu-repo/grantAgreement/ES/DGA/A07-20R info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es 3.231 2021 VETERINARY SCIENCES 16 / 145 = 0.11 2021 Q1 T1 AGRICULTURE, DAIRY & ANIMAL SCIENCE 13 / 62 = 0.21 2021 Q1 T1 0.61 2021 Veterinary (miscellaneous) 2021 Q1 Animal Science and Zoology 2021 Q1 2.7 2021 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Cardozo-Cerquera J.A. Grajales-Lombana H.A. Casao A. Universidad de Zaragoza (orcid)0000-0003-1997-4262 Pérez-Pe R. Universidad de Zaragoza (orcid)0000-0002-2312-6402 1002 819 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Zoología 11, 6 (2021), 1828 [16 pp.] Animals (Basel) Animals 2076-2615 1232511 http://zaguan.unizar.es/record/151376/files/texto_completo.pdf Versión publicada 2713873 http://zaguan.unizar.es/record/151376/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:151376 articulos driver 2025-10-17-14:25:05 ARTICLE