000151407 001__ 151407
000151407 005__ 20250307114716.0
000151407 0247_ $$2doi$$a10.1007/s11259-025-10679-y
000151407 0248_ $$2sideral$$a143128
000151407 037__ $$aART-2025-143128
000151407 041__ $$aeng
000151407 100__ $$aPérez, Estela$$uUniversidad de Zaragoza
000151407 245__ $$aModified aluminum (Al)-hematoxylin stain for detection of Al in sheep and cat tissues: an animal model for the study of Al-associated conditions
000151407 260__ $$c2025
000151407 5060_ $$aAccess copy available to the general public$$fUnrestricted
000151407 5203_ $$aAluminum (Al) has been an effective adjuvant in vaccines for nearly a century in animals and humans. Al can induce persistent granulomatous inflammatory responses characterized by macrophages with large granular, blue-gray cytoplasm sometimes with intracytoplasmic structures known as crystalloid bodies. These granulomas have been linked to proliferative and neoplastic conditions at injection sites in animal and humans. The detection of Al in tissue is crucial but current methods are time-intensive and require specialized training. This study highlights the efficacy of the modified Al-hematoxylin (MAH) technique in animals. Tissues from Al-induced lesions in sheep and cats were examined, along with non-aluminum granulomas as controls and MAH results were confirmed with lumogallion staining. In sheep, MAH positively stained granuloma macrophages, crystalloid bodies, necrotic regions and nearby connective tissue macrophages, while lymphoid aggregates present in granulomas were consistently negative. Macrophages loaded with Al and crystalloid bodies in lymph nodes were also MAH-positive, as were macrophages in contaminated injection sites following application of commercial Al-based vaccines. In cats with Feline Injection Site Sarcoma (FISS), MAH detected macrophage clusters around the tumor periphery and within the sarcoma. This cost-effective, rapid, and specific technique provides valuable insights for diagnosing Al-related conditions and could serve as an animal model for other species, including humans.
000151407 536__ $$9info:eu-repo/grantAgreement/ES/MCIU/FPU19-00553$$9info:eu-repo/grantAgreement/ES/MCIU/RTI2018-096172-B-C33
000151407 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000151407 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000151407 700__ $$aDiego, Alicia de
000151407 700__ $$aGómez, Álex$$uUniversidad de Zaragoza
000151407 700__ $$0(orcid)0000-0002-2685-1730$$aRodríguez-Largo, Ana
000151407 700__ $$0(orcid)0000-0002-8133-2124$$aPérez, Marta$$uUniversidad de Zaragoza
000151407 700__ $$0(orcid)0000-0002-2053-9842$$aLuján, Lluís$$uUniversidad de Zaragoza
000151407 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica
000151407 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000151407 7102_ $$11001$$2025$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Anatom.Anatom.Patológ.Com
000151407 773__ $$g49, 2 (2025), 108 [8 pp.]$$pVet. res. commun.$$tVeterinary Research Communications$$x0165-7380
000151407 8564_ $$s2609904$$uhttps://zaguan.unizar.es/record/151407/files/texto_completo.pdf$$yVersión publicada
000151407 8564_ $$s2387766$$uhttps://zaguan.unizar.es/record/151407/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000151407 909CO $$ooai:zaguan.unizar.es:151407$$particulos$$pdriver
000151407 951__ $$a2025-03-07-09:33:59
000151407 980__ $$aARTICLE