151428 20251017144651.0 doi 10.1002/smll.202412355 sideral 143131 ART-2025-143131 eng Bonet-Aleta, Javier (orcid)0000-0002-1791-0188 A Highly-Active Chemodynamic Agent Based on In Situ Generated Copper Complexes from Copper Hexacyanoferrate Nanoparticles 2025 Copper hexacyanoferrate (Cu2Fe(CN)6) nanocubes with a homogeneous size under 100 nm are synthesized by self‐assembly from Cu2+ and Fe(CN)63− precursors. Similar to previous reports with catalysts containing Cu and Fe, the objective is to produce a nanoparticle catalyst that can promote glutathione (GSH) oxidation thanks to the Cu contribution, plus some ROS production through Fenton‐like processes fostered by Fe. Unexpectedly, the catalytic activity for GSH oxidation are much higher (≈50%) than those obtained with equal Cu amounts provided as CuCl2. Furthermore, in the presence of GSH concentrations characteristic of the tumor microenvironment, the nanocubes disassembled homogeneously, without a noticeably change of composition. These results suggest that this strong increase of catalytic activity arises from synergistic coordination of the released Cu2+ and Fe(CN)63− ions that facilitate GSH deprotonation, accelerating its oxidation. Given the role of GSH in the nanoparticle disassembly process, a selective action of the catalyst can be obtained: lethal doses as low as 18 ppm of Cu are obtained for U251‐MG cancer cells while healthy fibroblasts are largely spared. info:eu-repo/grantAgreement/ES/AEI/CEX2023-001286-S info:eu-repo/grantAgreement/ES/DGA/E07-23R info:eu-repo/grantAgreement/ES/UZ-DGA/T57-23R info:eu-repo/grantAgreement/EC/H2020/742684/EU/Catalytic Dual-Function Devices Against Cancer/CADENCE This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 742684-CADENCE info:eu-repo/grantAgreement/ES/MICINN/PID2022-140159NA-I00 info:eu-repo/semantics/closedAccess All rights reserved http://www.europeana.eu/rights/rr-f/ info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Hueso, José L. Universidad de Zaragoza (orcid)0000-0002-4546-4111 Valls-Chiva, Ángeles Universidad de Zaragoza Ruiz-Aranda, Iris Manzanilla, Brenda García-Peiro, José I. Universidad de Zaragoza (orcid)0000-0003-4874-6672 Aina, Sergio Universidad de Zaragoza (orcid)0000-0002-2866-9369 Urriolabeitia, Esteban (orcid)0000-0001-9779-5820 Alegre-Requena, Juan V. (orcid)0000-0002-0769-7168 Santamaría, Jesús Universidad de Zaragoza (orcid)0000-0002-8701-9745 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 5005 790 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Tecnologi. Medio Ambiente 21, 13 (2025), 2412355 [14 pp.] Small Small 1613-6810 4167541 http://zaguan.unizar.es/record/151428/files/texto_completo.pdf Versión publicada 2312207 http://zaguan.unizar.es/record/151428/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:151428 articulos driver 2025-10-17-14:36:24 ARTICLE