000151446 001__ 151446
000151446 005__ 20250310131043.0
000151446 0247_ $$2doi$$a10.7554/eLife.94982
000151446 0248_ $$2sideral$$a143167
000151446 037__ $$aART-2024-143167
000151446 041__ $$aeng
000151446 100__ $$aSalgueiro-Toledo, Vivian C
000151446 245__ $$aMaintenance of cell wall remodeling and vesicle production are connected in Mycobacterium tuberculosis
000151446 260__ $$c2024
000151446 5060_ $$aAccess copy available to the general public$$fUnrestricted
000151446 5203_ $$aPathogenic and nonpathogenic mycobacteria secrete extracellular vesicles (EVs) under various conditions. EVs produced by Mycobacterium tuberculosis (Mtb) have raised significant interest for their potential in cell communication, nutrient acquisition, and immune evasion. However, the relevance of vesicle secretion during tuberculosis infection remains unknown due to the limited understanding of mycobacterial vesicle biogenesis. We have previously shown that a transposon mutant in the LCP-related gene virR (virRmut) manifested a strong attenuated phenotype during experimental macrophage and murine infections, concomitant to enhanced vesicle release. In this study, we aimed to understand the role of VirR in the vesicle production process in Mtb. We employ genetic, transcriptional, proteomics, ultrastructural, and biochemical methods to investigate the underlying processes explaining the enhanced vesiculogenesis phenomenon observed in the virRmut. Our results establish that VirR is critical to sustain proper cell permeability via regulation of cell envelope remodeling possibly through the interaction with similar cell envelope proteins, which control the link between peptidoglycan and arabinogalactan. These findings advance our understanding of mycobacterial extracellular vesicle biogenesis and suggest that these set of proteins could be attractive targets for therapeutic intervention.
000151446 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/PID2019-106859GA-I00
000151446 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000151446 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000151446 700__ $$aBertol, Jorge$$uUniversidad de Zaragoza
000151446 700__ $$aGutierrez, Claude
000151446 700__ $$aSerrano-Mestre, Jose L
000151446 700__ $$aFerrer-Luzon, Noelia
000151446 700__ $$aVázquez-Iniesta, Lucia
000151446 700__ $$aPalacios, Ainhoa
000151446 700__ $$aPasquina-Lemonche, Laia
000151446 700__ $$aEspaillat, Akbar
000151446 700__ $$aLerma, Laura
000151446 700__ $$aWeinrick, Brian
000151446 700__ $$aLavin, Jose L
000151446 700__ $$aElortza, Felix
000151446 700__ $$aAzkargorta, Mikel
000151446 700__ $$aPrieto, Alicia
000151446 700__ $$aBuendía-Nacarino, Pilar
000151446 700__ $$aLuque-García, Jose L
000151446 700__ $$aNeyrolles, Olivier
000151446 700__ $$aCava, Felipe
000151446 700__ $$aHobbs, Jamie K
000151446 700__ $$0(orcid)0000-0002-2980-9685$$aSanz, Joaquín$$uUniversidad de Zaragoza
000151446 700__ $$aPrados-Rosales, Rafael
000151446 7102_ $$12004$$2405$$aUniversidad de Zaragoza$$bDpto. Física Teórica$$cÁrea Física Teórica
000151446 773__ $$g13 (2024), [38 pp.]$$peLife (Cambridge)$$teLife$$x2050-084X
000151446 8564_ $$s4973351$$uhttps://zaguan.unizar.es/record/151446/files/texto_completo.pdf$$yVersión publicada
000151446 8564_ $$s2714730$$uhttps://zaguan.unizar.es/record/151446/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000151446 909CO $$ooai:zaguan.unizar.es:151446$$particulos$$pdriver
000151446 951__ $$a2025-03-10-12:55:43
000151446 980__ $$aARTICLE