000151533 001__ 151533
000151533 005__ 20251017144608.0
000151533 0247_ $$2doi$$a10.1016/j.xpro.2025.103643
000151533 0248_ $$2sideral$$a143165
000151533 037__ $$aART-2025-143165
000151533 041__ $$aeng
000151533 100__ $$aRabodoarivelo, Marie Sylvianne$$uUniversidad de Zaragoza
000151533 245__ $$aProtocol to quantify bacterial burden in time-kill assays using colony-forming units and most probable number readouts for Mycobacterium tuberculosis
000151533 260__ $$c2025
000151533 5060_ $$aAccess copy available to the general public$$fUnrestricted
000151533 5203_ $$aHere, we present a protocol to perform a time-kill assay (TKA) to quantify bacterial burden at multiple time points using colony-forming units and most probable number readouts simultaneously. We describe steps for preparing inoculum, experimental conditions, and sampling bacterial counts. We then detail procedures for quantification and analysis. TKAs provide longitudinal data reflecting the dynamics of the antibiotic effect over time against a planktonic culture and quantify the concentration-effect relationship.Here, we present a protocol to perform a time-kill assay (TKA) to quantify bacterial burden at multiple time points using colony-forming units and most probable number readouts simultaneously. We describe steps for preparing inoculum, experimental conditions, and sampling bacterial counts. We then detail procedures for quantification and analysis. TKAs provide longitudinal data reflecting the dynamics of the antibiotic effect over time against a planktonic culture and quantify the concentration-effect relationship.
000151533 536__ $$9info:eu-repo/grantAgreement/EC/H2020/853989/EU/EUROPEAN REGIMEN ACCELERATOR FOR TUBERCULOSIS/ERA4TB$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 853989-ERA4TB
000151533 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
000151533 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000151533 700__ $$aHoffmann, Eik
000151533 700__ $$aGaudin, Cyril
000151533 700__ $$aAguilar-Ayala, Diana Angélica$$uUniversidad de Zaragoza
000151533 700__ $$aGalizia, Jordana$$uUniversidad de Zaragoza
000151533 700__ $$aSonnenkalb, Lindsay
000151533 700__ $$aDal Molin, Michael
000151533 700__ $$aCioetto-Mazzabò, Laura
000151533 700__ $$aDegiacomi, Giulia
000151533 700__ $$aRecchia, Deborah
000151533 700__ $$aRybniker, Jan
000151533 700__ $$aManganelli, Riccardo
000151533 700__ $$aPasca, Maria Rosalia
000151533 700__ $$0(orcid)0000-0002-8480-0325$$aRamón-García, Santiago
000151533 700__ $$0(orcid)0000-0002-0111-4697$$aLucía, Ainhoa$$uUniversidad de Zaragoza
000151533 7102_ $$11011$$2630$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cÁrea Microbiología
000151533 773__ $$g6, 1 (2025), 103643 [18 pp.]$$tSTAR Protocols$$x2666-1667
000151533 8564_ $$s5101162$$uhttps://zaguan.unizar.es/record/151533/files/texto_completo.pdf$$yVersión publicada
000151533 8564_ $$s1471029$$uhttps://zaguan.unizar.es/record/151533/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000151533 909CO $$ooai:zaguan.unizar.es:151533$$particulos$$pdriver
000151533 951__ $$a2025-10-17-14:15:59
000151533 980__ $$aARTICLE