151533 20251017144608.0 doi 10.1016/j.xpro.2025.103643 sideral 143165 ART-2025-143165 eng Rabodoarivelo, Marie Sylvianne Universidad de Zaragoza Protocol to quantify bacterial burden in time-kill assays using colony-forming units and most probable number readouts for Mycobacterium tuberculosis 2025 Access copy available to the general public Unrestricted Here, we present a protocol to perform a time-kill assay (TKA) to quantify bacterial burden at multiple time points using colony-forming units and most probable number readouts simultaneously. We describe steps for preparing inoculum, experimental conditions, and sampling bacterial counts. We then detail procedures for quantification and analysis. TKAs provide longitudinal data reflecting the dynamics of the antibiotic effect over time against a planktonic culture and quantify the concentration-effect relationship.Here, we present a protocol to perform a time-kill assay (TKA) to quantify bacterial burden at multiple time points using colony-forming units and most probable number readouts simultaneously. We describe steps for preparing inoculum, experimental conditions, and sampling bacterial counts. We then detail procedures for quantification and analysis. TKAs provide longitudinal data reflecting the dynamics of the antibiotic effect over time against a planktonic culture and quantify the concentration-effect relationship. info:eu-repo/grantAgreement/EC/H2020/853989/EU/EUROPEAN REGIMEN ACCELERATOR FOR TUBERCULOSIS/ERA4TB This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 853989-ERA4TB info:eu-repo/semantics/openAccess by-nc-nd https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Hoffmann, Eik Gaudin, Cyril Aguilar-Ayala, Diana Angélica Universidad de Zaragoza Galizia, Jordana Universidad de Zaragoza Sonnenkalb, Lindsay Dal Molin, Michael Cioetto-Mazzabò, Laura Degiacomi, Giulia Recchia, Deborah Rybniker, Jan Manganelli, Riccardo Pasca, Maria Rosalia Ramón-García, Santiago (orcid)0000-0002-8480-0325 Lucía, Ainhoa Universidad de Zaragoza (orcid)0000-0002-0111-4697 1011 630 Universidad de Zaragoza Dpto. Microb.Ped.Radio.Sal.Pú. Área Microbiología 6, 1 (2025), 103643 [18 pp.] STAR Protocols 2666-1667 5101162 http://zaguan.unizar.es/record/151533/files/texto_completo.pdf Versión publicada 1471029 http://zaguan.unizar.es/record/151533/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:151533 articulos driver 2025-10-17-14:15:59 ARTICLE