000151544 001__ 151544 000151544 005__ 20250313085758.0 000151544 0247_ $$2doi$$a10.1021/acsagscitech.4c00338 000151544 0248_ $$2sideral$$a143199 000151544 037__ $$aART-2025-143199 000151544 041__ $$aeng 000151544 100__ $$0(orcid)0000-0002-1532-3916$$aBen Jeddou, Khaoula 000151544 245__ $$aEvaluation of the Accumulation, Distribution, and Excretion of Different Silver Species in Tissues and Feces from Chickens and Pigs Fed with Silver-Based Nanomaterial Supplemented Feeds 000151544 260__ $$c2025 000151544 5203_ $$aOn the basis of two in vivo experiments involving pigs and chickens, where animals were fed with feeds supplemented with a silver–kaolin nanomaterial as a growth promoter, the fate of the ingested silver, its accumulation in different tissues and excretion, and its occurrence as ionic or particulate forms has been studied. Inductively coupled plasma mass spectrometry (ICP-MS) in conventional mode was used for the quantitative determination of total silver in the different tissues and feces. Results showed that silver accumulated in liver (0.15–5 mg kg–1) and kidney (0.4 mg kg–1) but not in muscle, while the major part of silver was eliminated through the feces (75–1026 mg kg–1). Laser ablation coupled to ICP-MS (LA-ICP-MS) in a conventional mode revealed a preferential accumulation of silver in the outer layer of the liver lobules in pigs. LA-ICP-MS in single particle mode (LA-SP-ICP-MS) was applied to the analysis of tissues and feces to obtain speciation information about the presence of ionic and particulate silver in the samples. Silver found in the pig liver was present in ionic form, confirming that silver was absorbed in the intestine in this form. The analysis of pig feces confirmed the presence of both ionic silver and particles containing silver with average masses of 520 ag (equivalent to solid spherical silver nanoparticles of 50 nm diameter). 000151544 536__ $$9info:eu-repo/grantAgreement/ES/DGA-FEDER/E29-23R$$9info:eu-repo/grantAgreement/EC/H2020/801586/EU/International Doctoral Programme for Talent Attraction to the Campus of International Excellence of the Ebro Valley/IberusTalent$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 801586-IberusTalent$$9info:eu-repo/grantAgreement/ES/MICINN/PID2021-123203OB-I00 000151544 540__ $$9info:eu-repo/semantics/closedAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/ 000151544 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000151544 700__ $$0(orcid)0000-0002-8066-7117$$aBakir, Mariam 000151544 700__ $$aJimenez, Maria S. 000151544 700__ $$0(orcid)0000-0002-0712-1185$$aFondevila, Manuel$$uUniversidad de Zaragoza 000151544 700__ $$aMetarapi, Dino 000151544 700__ $$aŠala, Martin 000151544 700__ $$avan Elteren, Johannes T. 000151544 700__ $$0(orcid)0000-0002-4169-0357$$aLaborda, Francisco$$uUniversidad de Zaragoza 000151544 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica 000151544 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal 000151544 773__ $$tACS Agricultural Science and Technology$$x2692-1952 000151544 8564_ $$s2543593$$uhttps://zaguan.unizar.es/record/151544/files/texto_completo.pdf$$yVersión publicada 000151544 8564_ $$s3124087$$uhttps://zaguan.unizar.es/record/151544/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000151544 909CO $$ooai:zaguan.unizar.es:151544$$particulos$$pdriver 000151544 951__ $$a2025-03-13-08:39:41 000151544 980__ $$aARTICLE