000151551 001__ 151551
000151551 005__ 20250313085758.0
000151551 0247_ $$2doi$$a10.1139/bcb-2024-0087
000151551 0248_ $$2sideral$$a142620
000151551 037__ $$aART-2025-142620
000151551 041__ $$aeng
000151551 100__ $$aAbad, Inés
000151551 245__ $$aLactoferrin modulates oxidative stress and inflammatory cytokines in a murine model of dysbiosis induced by clindamycin
000151551 260__ $$c2025
000151551 5060_ $$aAccess copy available to the general public$$fUnrestricted
000151551 5203_ $$aAntibiotics, specifically clindamycin (Clin), cause intestinal dysbiosis, reducing the microbiota with anti-inflammatory properties. Furthermore, Clin can induce alterations in the immune responses and oxidative stress. Lactoferrin, among other activities, participates in the maintenance of intestinal homeostasis and reduces dysbiosis induced by antibiotic treatment. The aim of this study was to analyze the effect of native and iron-saturated bovine LF in a murine model of dysbiosis induced by Clin. Six groups of male C57BL/6 mice were treated with saline (control), Clin, native lactoferrin (nLF), iron-saturated lactoferrin (sLF), nLF/Clin, or sLF/Clin. Oxidation caused in the intestinal cells of the ileum of animals subjected to different treatments was analyzed, focusing on lipid peroxidation and protein carbonyl content. The expression of inflammatory mediators was determined by qRT-PCR. Treatment with Clin did not modify lipid peroxidation, but significantly increased protein carbonyl levels up to almost 5-fold respect to the control, an effect that was reversed by orally administering sLF to mice. Furthermore, Clin increased the expression of interleukin-6 and TNF-α by 1- and 2-fold change, respectively. This effect was reversed by treatment with nLF and sLF, decreasing the expression to basal levels. In conclusion, this study indicates that lactoferrin can prevent some of the effects of Clin on intestinal cells and their associated immune system.
000151551 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000151551 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000151551 700__ $$0(orcid)0000-0002-6546-6149$$aBellés, Andrea
000151551 700__ $$0(orcid)0000-0002-2685-1730$$aRodríguez-Largo, Ana
000151551 700__ $$0(orcid)0000-0002-2053-9842$$aLuján, Lluís$$uUniversidad de Zaragoza
000151551 700__ $$0(orcid)0000-0002-1204-4356$$ade Blas, Ignacio$$uUniversidad de Zaragoza
000151551 700__ $$aGraikini, Dimitra
000151551 700__ $$0(orcid)0000-0002-5306-9365$$aGrasa, Laura$$uUniversidad de Zaragoza
000151551 700__ $$0(orcid)0000-0001-5964-823X$$aSánchez, Lourdes$$uUniversidad de Zaragoza
000151551 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000151551 7102_ $$11012$$2410$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Fisiología
000151551 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos
000151551 773__ $$g103 (2025), 1-12$$pBiochem. cell. biol.$$tBIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE$$x0829-8211
000151551 8564_ $$s952234$$uhttps://zaguan.unizar.es/record/151551/files/texto_completo.pdf$$yPostprint
000151551 8564_ $$s1761253$$uhttps://zaguan.unizar.es/record/151551/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000151551 909CO $$ooai:zaguan.unizar.es:151551$$particulos$$pdriver
000151551 951__ $$a2025-03-13-08:39:50
000151551 980__ $$aARTICLE