151645 20251017144619.0 doi 10.3390/molecules26082105 sideral 126644 ART-2021-126644 eng Lira-Navarrete E. Universidad de Zaragoza Protein o-fucosyltransferase 1 undergoes interdomain flexibility in solution 2021 Access copy available to the general public Unrestricted Protein O-fucosyltransferase 1 (PoFUT1) is a GT-B fold enzyme that fucosylates proteins containing EGF-like repeats. GT-B glycosyltransferases have shown a remarkable grade of plasticity adopting closed and open conformations as a way of tuning their catalytic cycle, a feature that has not been observed for PoFUT1. Here, we analyzed Caenorhabditis elegans PoFUT1 (CePoFUT1) conformational behavior in solution by atomic force microscopy (AFM) and single-molecule fluorescence resonance energy transfer (SMF-FRET). Our results show that this enzyme is very flexible and adopts mainly compact conformations and to a lesser extend a highly dynamic population that oscillates between compact and highly extended conformations. Overall, our experiments illustrate the inherent complexity of CePoFUT1 dynamics, which might play a role during its catalytic cycle. info:eu-repo/grantAgreement/ES/DGA-FEDER/E35-20R info:eu-repo/grantAgreement/ES/DGA/LMP58-18 info:eu-repo/grantAgreement/ES/MEC/BFU2016-75633-P info:eu-repo/grantAgreement/ES/MEC/CTQ2013-44367-C2-2-P info:eu-repo/grantAgreement/ES/MEC/PID2019-105451GB-I00 info:eu-repo/grantAgreement/ES/MICINN/IJCI-2017-32874 info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es 4.927 2021 CHEMISTRY, MULTIDISCIPLINARY 65 / 179 = 0.363 2021 Q2 T2 BIOCHEMISTRY & MOLECULAR BIOLOGY 114 / 297 = 0.384 2021 Q2 T2 0.705 2021 Analytical Chemistry 2021 Q1 Drug Discovery 2021 Q1 Pharmaceutical Science 2021 Q1 Molecular Medicine 2021 Q1 Organic Chemistry 2021 Q1 Medicine (miscellaneous) 2021 Q1 5.9 2021 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pallarés M.C. Castello F. Ruedas-Rama M.J. Orte A. Gracia Lostao A. (orcid)0000-0001-7460-5916 Hurtado-Guerrero R. (orcid)0000-0002-3122-9401 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 26, 8 (2021), 2105 [14 pp.] Molecules (Basel, Online) Molecules 1420-3049 3532770 http://zaguan.unizar.es/record/151645/files/texto_completo.pdf Versión publicada 2769142 http://zaguan.unizar.es/record/151645/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:151645 articulos driver 2025-10-17-14:21:08 ARTICLE