151682 20251017144630.0 doi 10.1016/j.postharvbio.2025.113512 sideral 143255 ART-2025-143255 eng Esteban-Sanz, Clara Universidad de Zaragoza (orcid)0009-0005-0336-7582 Variety, maturity stage and postharvest storage time effect on Mal d 3 allergen from apple (Malus domestica) 2025 Access copy available to the general public Unrestricted Fruit belonging to Rosaceae family, which includes apples, is responsible for 60 % of fruit allergies. Mal d 3, a member of the lipid transfer proteins, is the most allergenic protein of apple. Levels of total soluble protein and Mal d 3 were determined in apple peel as a function of variety, maturity stage and postharvest storage time under controlled atmosphere conditions, using a previously developed specific sandwich ELISA. Mal d 3 content differed among varieties, ranging from 13.6 mg kg−1 in ‘Golden Delicious’ to 23.6 mg kg−1 in ‘Verde Doncella’. No significant influence of the maturity stage on Mal d 3 concentration was found. Over a 7-month period of storage under controlled atmosphere, the levels of Mal d 3 decreased about 1.6–2.3 times. No correlation was found between Mal d 3 concentration and total soluble protein in apple peel samples. A better understanding of how Mal d 3 concentration is affected by pre and postharvest factors could assist to decrease the allergen load of apple. info:eu-repo/grantAgreement/ES/DGA/A20-23R info:eu-repo/semantics/embargoedAccess by-nc-nd https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Tobajas, Ana P. Universidad de Zaragoza (orcid)0000-0002-2646-5733 Gracia, Ana P. Universidad de Zaragoza (orcid)0000-0001-9333-3055 Civera, Alba (orcid)0000-0001-7719-6388 Sánchez, Lourdes Universidad de Zaragoza (orcid)0000-0001-5964-823X Arias, Esther Universidad de Zaragoza (orcid)0000-0002-4843-2561 Pérez, María D. Universidad de Zaragoza (orcid)0000-0003-2555-8425 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 225 (2025), 113512 [8 pp.] Postharvest biol. technol. Postharvest Biology and Technology 0925-5214 718391 http://zaguan.unizar.es/record/151682/files/texto_completo.pdf Postprint info:eu-repo/date/embargoEnd/2027-03-13 587603 http://zaguan.unizar.es/record/151682/files/texto_completo.jpg?subformat=icon icon Postprint info:eu-repo/date/embargoEnd/2027-03-13 oai:zaguan.unizar.es:151682 articulos driver 2025-10-17-14:25:52 ARTICLE