000151716 001__ 151716 000151716 005__ 20250319155218.0 000151716 0247_ $$2doi$$a10.1016/j.jddst.2025.106749 000151716 0248_ $$2sideral$$a143252 000151716 037__ $$aART-2025-143252 000151716 041__ $$aeng 000151716 100__ $$aZiani, Kaoutar 000151716 245__ $$aHyaluronic acid incorporation into hybrid alginate-based bioinks promotes long term viability and controlled release of 3D embedded scaffolds mesenchymal stem cells in absence of Dimethyl Sulfoxide cryoprotectant 000151716 260__ $$c2025 000151716 5203_ $$aCryopreservation is critical in 3D bioprinting to ensure availability for clinical use and long-term storage. We have studied the incorporation of hyaluronic acid (Ha) into an alginate-nanocellulose bioink to enhance scaffold properties and reduce reliance on dimethyl sulfoxide (DMSO), a traditional, but toxic cryoprotectant. Our findings show that Ha significantly improves biomaterial ink viscoelasticity, even after cryopreservation at ultra-low temperatures. Moreover, scaffolds incorporating Ha exhibited increased roughness and porosity and mechanical testing indicated that cryopreserved scaffolds with Ha were more durable. Interestingly, scaffolds without DMSO maintained high cell viability and metabolic activity, suggesting effective cryoprotection with reduced cytotoxicity. In conclusion, incorporating Ha into bioinks enhances scaffold properties and enables successful cryopreservation without DMSO, paving the way for safer and more efficient bioinks in tissue engineering and regenerative medicine. 000151716 540__ $$9info:eu-repo/semantics/closedAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/ 000151716 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000151716 700__ $$aPlou, Javier 000151716 700__ $$aSaenz-del-Burgo, Laura 000151716 700__ $$aGarcía, Isabel 000151716 700__ $$0(orcid)0000-0002-8666-622X$$aCiriza, Jesús$$uUniversidad de Zaragoza 000151716 700__ $$aPedraz, Jose Luis 000151716 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Histología 000151716 773__ $$g106 (2025), 106749 [10 pp.]$$pJ. drug deliv. sci. technol.$$tJOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY$$x1773-2247 000151716 8564_ $$s6181120$$uhttps://zaguan.unizar.es/record/151716/files/texto_completo.pdf$$yVersión publicada 000151716 8564_ $$s2508039$$uhttps://zaguan.unizar.es/record/151716/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000151716 909CO $$ooai:zaguan.unizar.es:151716$$particulos$$pdriver 000151716 951__ $$a2025-03-19-14:21:24 000151716 980__ $$aARTICLE