151716 20251017144640.0 doi 10.1016/j.jddst.2025.106749 sideral 143252 ART-2025-143252 eng Ziani, Kaoutar Hyaluronic acid incorporation into hybrid alginate-based bioinks promotes long term viability and controlled release of 3D embedded scaffolds mesenchymal stem cells in absence of Dimethyl Sulfoxide cryoprotectant 2025 Cryopreservation is critical in 3D bioprinting to ensure availability for clinical use and long-term storage. We have studied the incorporation of hyaluronic acid (Ha) into an alginate-nanocellulose bioink to enhance scaffold properties and reduce reliance on dimethyl sulfoxide (DMSO), a traditional, but toxic cryoprotectant. Our findings show that Ha significantly improves biomaterial ink viscoelasticity, even after cryopreservation at ultra-low temperatures. Moreover, scaffolds incorporating Ha exhibited increased roughness and porosity and mechanical testing indicated that cryopreserved scaffolds with Ha were more durable. Interestingly, scaffolds without DMSO maintained high cell viability and metabolic activity, suggesting effective cryoprotection with reduced cytotoxicity. In conclusion, incorporating Ha into bioinks enhances scaffold properties and enables successful cryopreservation without DMSO, paving the way for safer and more efficient bioinks in tissue engineering and regenerative medicine. info:eu-repo/semantics/closedAccess All rights reserved http://www.europeana.eu/rights/rr-f/ info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Plou, Javier Saenz-del-Burgo, Laura García, Isabel Ciriza, Jesús Universidad de Zaragoza (orcid)0000-0002-8666-622X Pedraz, Jose Luis 1003 443 Universidad de Zaragoza Dpto. Anatom.Histolog.Humanas Area Histología 106 (2025), 106749 [10 pp.] J. drug deliv. sci. technol. JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY 1773-2247 6181120 http://zaguan.unizar.es/record/151716/files/texto_completo.pdf Versión publicada 2508039 http://zaguan.unizar.es/record/151716/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:151716 articulos driver 2025-10-17-14:31:27 ARTICLE