000151964 001__ 151964 000151964 005__ 20251017144614.0 000151964 0247_ $$2doi$$a10.3389/fmicb.2021.614243 000151964 0248_ $$2sideral$$a126172 000151964 037__ $$aART-2021-126172 000151964 041__ $$aeng 000151964 100__ $$aAragón-Aranda B. 000151964 245__ $$aThe Phospholipid N-Methyltransferase and Phosphatidylcholine Synthase Pathways and the ChoXWV Choline Uptake System Involved in Phosphatidylcholine Synthesis Are Widely Conserved in Most, but Not All Brucella Species 000151964 260__ $$c2021 000151964 5060_ $$aAccess copy available to the general public$$fUnrestricted 000151964 5203_ $$aThe brucellae are facultative intracellular bacteria with a cell envelope rich in phosphatidylcholine (PC). PC is abundant in eukaryotes but rare in prokaryotes, and it has been proposed that Brucella uses PC to mimic eukaryotic-like features and avoid innate immune responses in the host. Two PC synthesis pathways are known in prokaryotes: the PmtA-catalyzed trimethylation of phosphatidylethanolamine and the direct linkage of choline to CDP-diacylglycerol catalyzed by the PC synthase Pcs. Previous studies have reported that B. abortus and B. melitensis possess non-functional PmtAs and that PC is synthesized exclusively via Pcs in these strains. A putative choline transporter ChoXWV has also been linked to PC synthesis in B. abortus. Here, we report that Pcs and Pmt pathways are active in B. suis biovar 2 and that a bioinformatics analysis of Brucella genomes suggests that PmtA is only inactivated in B. abortus and B. melitensis strains. We also show that ChoXWV is active in B. suis biovar 2 and conserved in all brucellae except B. canis and B. inopinata. Unexpectedly, the experimentally verified ChoXWV dysfunction in B. canis did not abrogate PC synthesis in a PmtA-deficient mutant, which suggests the presence of an unknown mechanism for obtaining choline for the Pcs pathway in Brucella. We also found that ChoXWV dysfunction did not cause attenuation in B. suis biovar 2. The results of these studies are discussed with respect to the proposed role of PC in Brucella virulence and how differential use of the Pmt and Pcs pathways may influence the interactions of these bacteria with their mammalian hosts. 000151964 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A13-17R$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2014-58795-C4-1-R$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2014-58795-C4-3-R$$9info:eu-repo/grantAgreement/ES/MINECO/PID2019-107601RA-C32 000151964 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es 000151964 590__ $$a6.064$$b2021 000151964 591__ $$aMICROBIOLOGY$$b34 / 137 = 0.248$$c2021$$dQ1$$eT1 000151964 592__ $$a1.314$$b2021 000151964 593__ $$aMicrobiology (medical)$$c2021$$dQ1 000151964 593__ $$aMicrobiology$$c2021$$dQ1 000151964 594__ $$a8.2$$b2021 000151964 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000151964 700__ $$aPalacios-Chaves L. 000151964 700__ $$aSalvador-Bescós M. 000151964 700__ $$aMiguel M.J. de 000151964 700__ $$0(orcid)0000-0002-7970-4929$$aMuñoz P.M. 000151964 700__ $$aVences-Guzmán M.Á. 000151964 700__ $$aZúñiga-Ripa A. 000151964 700__ $$aLázaro-Antón L. 000151964 700__ $$aSohlenkamp C. 000151964 700__ $$aMoriyón I. 000151964 700__ $$aIriarte M. 000151964 700__ $$aConde-Álvarez R. 000151964 773__ $$g12 (2021), 614243 [12 pp.]$$pFront. microbiol.$$tFrontiers in Microbiology$$x1664-302X 000151964 8564_ $$s958189$$uhttps://zaguan.unizar.es/record/151964/files/texto_completo.pdf$$yVersión publicada 000151964 8564_ $$s2371082$$uhttps://zaguan.unizar.es/record/151964/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000151964 909CO $$ooai:zaguan.unizar.es:151964$$particulos$$pdriver 000151964 951__ $$a2025-10-17-14:18:31 000151964 980__ $$aARTICLE