152081 20251017144622.0 doi 10.1128/aem.01829-24 sideral 143394 ART-2025-143394 eng Ivanova, Mirena Large-scale phenotypic and genomic analysis of Listeria monocytogenes reveals diversity in the sensitivity to quaternary ammonium compounds but not to peracetic acid 2025 Access copy available to the general public Unrestricted Listeria monocytogenes presents a significant concern for the food industry due to its ability to persist in the food processing environment. One of the factors contributing to its persistence is decreased sensitivity to disinfectants. Our objective was to assess the diversity of L. monocytogenes sensitivity to food industry disinfectants by testing the response of 1,671 L. monocytogenes isolates to quaternary ammonium compounds (QACs) and 414 isolates to peracetic acid (PAA) using broth microdilution and growth curve analysis assays, respectively, and to categorize the isolates into sensitive and tolerant. A high phenotype-genotype concordance (95%) regarding tolerance to QACs was obtained by screening the genomes for the presence of QAC tolerance-associated genes bcrABC, emrE, emrC, and qacH. Based on this high concordance, we assessed the QAC genes’ dissemination among publicly available L. monocytogenes genomes (n = 39,196). Overall, QAC genes were found in 23% and 28% of the L. monocytogenes collection in this study and in the global data set, respectively. bcrABC and qacH were the most prevalent genes, with bcrABC being the most detected QAC gene in the USA, while qacH dominated in Europe. No significant differences (P > 0.05) in the PAA tolerance were detected among isolates belonging to different lineages, serogroups, clonal complexes, or isolation sources, highlighting limited variation in the L. monocytogenes sensitivity to this disinfectant. The present work represents the largest testing of L. monocytogenes sensitivity to important food industry disinfectants at the phenotypic and genomic level, revealing diversity in the tolerance to QACs while all isolates showed similar sensitivity to PAA. info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Laage Kragh, Martin Szarvas, Judit Tosun, Elif Seyda Holmud, Natacha Friis Gmeiner, Alexander Amar, Corinne Guldimann, Claudia Huynh, TuAnh N. Karpíšková, Renáta Rota, Carmen Universidad de Zaragoza (orcid)0000-0001-6375-9784 Gomez, Diego Universidad de Zaragoza (orcid)0000-0001-6194-8346 Aboagye, Eurydice Etter, Andrea Centorame, Patrizia Torresi, Marina De Angelis, Maria Elisabetta Pomilio, Francesco Okholm, Anders Hauge Xiao, Yinghua Kleta, Sylvia Lüth, Stefanie Pietzka, Ariane Kovacevic, Jovana Pagotto, Franco Rychli, Kathrin Zdovc, Irena Papic, Bojan Heir, Even Langsrud, Solveig Møretrø, Trond Brown, Phillip Kathariou, Sophia Stephan, Roger Tasara, Taurai Dalgaard, Paw Njage, Patrick Murigu Kamau Fagerlund, Annette Aarestrup, Frank Truelstrup Hansen, Lisbeth Leekitcharoenphon, Pimlapas 2008 640 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Nutrición Bromatología Applied and Environmental Microbiology 0099-2240 Raw sequencing data https://www.ebi.ac.uk/ena/browser/view/PRJEB56155 6471389 http://zaguan.unizar.es/record/152081/files/texto_completo.pdf Versión publicada 2541265 http://zaguan.unizar.es/record/152081/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:152081 articulos driver 2025-10-17-14:22:16 ARTICLE