000152095 001__ 152095
000152095 005__ 20250326144155.0
000152095 0247_ $$2doi$$a10.1002/mbo3.1234
000152095 0248_ $$2sideral$$a126530
000152095 037__ $$aART-2021-126530
000152095 041__ $$aeng
000152095 100__ $$aDresen, M.
000152095 245__ $$aIdentification and characterization of the cell division protein MapZ from Streptococcus suis
000152095 260__ $$c2021
000152095 5060_ $$aAccess copy available to the general public$$fUnrestricted
000152095 5203_ $$aStreptococcus suis, an emerging zoonotic pathogen, causes invasive diseases in pigs, including sepsis, meningitis, endocarditis, pneumonia, and arthritis. Importantly, similar pathologies are reported in human S. suis infections. In previous work, the locus SSU0375 of S. suis strain P1.7 had been identified as a conditionally essential gene by intrathecal experimental infection of pigs with a transposon library of S. suis. This study aimed to identify the function of the corresponding gene product. Bioinformatics analysis and homology modeling revealed sequence and structural homologies with the Streptococcus pneumoniae mid-cell-anchored protein Z (MapZ) that is involved in cell division in different bacterial species. Indeed, depletion of this locus in S. suis strain 10 revealed a growth defect as compared to the wild type. Electron microscopy analysis of the corresponding mutant demonstrated morphological growth defects as compared to the wild-type strain, including an irregular cell shape and size as well as mispositioned division septa. Light microscopy and subsequent quantitative image analysis confirmed these morphological alterations. In the genetic rescue strain, the wild-type phenotype was completely restored. In summary, we proposed that SSU0375 or the corresponding locus in strain 10 encode for a S. suis MapZ homolog that guides septum positioning as evidenced for other members of the Streptococci family.
000152095 536__ $$9info:eu-repo/grantAgreement/EC/H2020/727966/EU/Program for Innovative Global Prevention of Streptococcus suis/PIGSs$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 727966-PIGSs
000152095 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttp://creativecommons.org/licenses/by-nc/3.0/es/
000152095 590__ $$a3.904$$b2021
000152095 591__ $$aMICROBIOLOGY$$b73 / 137 = 0.533$$c2021$$dQ3$$eT2
000152095 592__ $$a0.711$$b2021
000152095 593__ $$aMicrobiology$$c2021$$dQ3
000152095 594__ $$a5.9$$b2021
000152095 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000152095 700__ $$aRohde, M.
000152095 700__ $$0(orcid)0000-0002-8134-0693$$aArenas, J.$$uUniversidad de Zaragoza
000152095 700__ $$aGreeff, A. de
000152095 700__ $$aNerlich, A.
000152095 700__ $$aValentin-Weigand, P.
000152095 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000152095 773__ $$g10, 5 (2021), e1234 [16 pp.]$$pMicrobiologyOpen$$tMicrobiologyOpen$$x2045-8827
000152095 8564_ $$s2262055$$uhttps://zaguan.unizar.es/record/152095/files/texto_completo.pdf$$yVersión publicada
000152095 8564_ $$s2273863$$uhttps://zaguan.unizar.es/record/152095/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000152095 909CO $$ooai:zaguan.unizar.es:152095$$particulos$$pdriver
000152095 951__ $$a2025-03-26-13:55:03
000152095 980__ $$aARTICLE