000152311 001__ 152311 000152311 005__ 20250401114420.0 000152311 037__ $$aTAZ-TFM-2024-1537 000152311 041__ $$aeng 000152311 1001_ $$aAdwan Al Chihabi, Lara 000152311 24200 $$aModel cell membranes for biomedical applications. 000152311 24500 $$aModelos de membrana celular para aplicaciones biomédicas. 000152311 260__ $$aZaragoza$$bUniversidad de Zaragoza$$c2024 000152311 506__ $$aby-nc-sa$$bCreative Commons$$c3.0$$uhttp://creativecommons.org/licenses/by-nc-sa/3.0/ 000152311 520__ $$aAnalysing the interactions of xenobiotics, pharmaceutical agents, nanoparticles, or other agents with cell membranes is a challenging process, largely due to the structural and functional complexity of cell membranes. To overcome these challenges, fundamental research often employs artificially assembled model cell membranes, which allow precise control over their composition and properties. In this Final Master’s thesis, the Langmuir technique is used to model the plasma membrane of healthy and inflammatory bowel disease-affected colonocytes at the air-water interface. These model membranes are used to investigate their interactions with [6]-Gingerol, a xenobiotic well-known for its antioxidant, anti-inflammatory, and antitumoral properties as well as its protective role in patients with inflammatory bowel disease (IBD). However, the low water solubility of [6]-Gingerol severely restricts its bioavailability, and thus its use as a therapeutic agent. This Final Master’s objective is to gather information about the molecular interactions between [6]-Gingerol and the components of plasma membranes, which could lay the groundwork for future development of delivery systems (e.g. liposomes or artificial exosomes) aimed at enhancing the therapeutic potential of [6]-Gingerol. In addition, the analysis of the interactions of [6]-Gingerol with healthy and disturbed model cell membranes could also contribute to a better understanding of the therapeutic effect of this xenobiotic. The influence of [6]-Gingerol in membrane fluidity, stability, and thermodynamic properties was assessed through the data retrieved from surface pressure-area per molecule isotherms. Results indicate that higher χGIN values increase membrane fluidity. From a thermodynamic point of view, both systems exhibit repulsive interactions between [6]-Gingerol and membrane components, which are generally stronger in the healthy membrane (HM). Incorporation of [6]-Gingerol into both model membranes was also studied using adsorption experiments in which surface pressure measurements were analysed, and by means of the in situ visualization of the monolayers at the air-water interface using Brewster Angle Microscopy (BAM). In conclusion, [6]-Gingerol is capable of penetrating both model membranes, exhibiting a more favourable incorporation in the HM membrane as opposed to the IBD-affected one. All these findings highlight the distinct thermodynamic and structural behaviour of [6]-Gingerol interactions with healthy versus IBD-affected membrane models, contributing to a deeper understanding of the impact of [6]-Gingerol on plasma membrane properties.<br /><br /> 000152311 521__ $$aMáster en Materiales Nanoestructurados para Aplicaciones Nanotecnológicas (Nanostructured Materials for Nanotechnology Applications) 000152311 540__ $$aDerechos regulados por licencia Creative Commons 000152311 691__ $$a9 000152311 692__ $$aCon este TFM, las conclusiones obtenidas de las interacciones de [6]-Gingerol con modelos de membrana sienta las bases para posibles futuros desarrollos de nuevos sistemas de distribución del mismo, con el fin de mejorar el potencial terapéutico de [6]-Gingerol. 000152311 700__ $$aCea Mingueza, Pilar$$edir. 000152311 700__ $$aMartín Solans, Santiago$$edir. 000152311 7102_ $$aUniversidad de Zaragoza$$b $$c 000152311 8560_ $$f780647@unizar.es 000152311 8564_ $$s2877325$$uhttps://zaguan.unizar.es/record/152311/files/TAZ-TFM-2024-1537.pdf$$yMemoria (eng) 000152311 909CO $$ooai:zaguan.unizar.es:152311$$pdriver$$ptrabajos-fin-master 000152311 950__ $$a 000152311 951__ $$adeposita:2025-04-01 000152311 980__ $$aTAZ$$bTFM$$cCIEN 000152311 999__ $$a20241121213409.CREATION_DATE