000152831 001__ 152831 000152831 005__ 20250401114425.0 000152831 037__ $$aTAZ-TFG-2024-2345 000152831 041__ $$aeng 000152831 1001_ $$aGordo Ortiz, Andrés 000152831 24200 $$aAssessing the phenotype of Pancreatic Cancer Stem Cells under adaptation to Serine and Glycine deficiency: Effects on Metabolic Plasticity and Immune Evasive properties 000152831 24500 $$aEvaluación del fenotipo de las células madre tumorales de páncreas en la adaptación a la deficiencia de serina y glicina: efectos en la plasticidad metabólica y la evasión inmune 000152831 260__ $$aZaragoza$$bUniversidad de Zaragoza$$c2024 000152831 506__ $$aby-nc-sa$$bCreative Commons$$c3.0$$uhttp://creativecommons.org/licenses/by-nc-sa/3.0/ 000152831 520__ $$aPancreatic Ductal Adenocarcinoma remains a main cause of cancer-related deaths due to late diagnosis and limited effective treatments. Large regions in pancreatic cancer tissues are hypoxic and nutrient-deprived because of unregulated cell growth and insufficient vascular supply. However, certain cancer cells, such as cancer stem cells (CSCs), can tolerate these severe conditions and survive for prolonged periods. Although many studies have addressed this adaptative behaviour in a myriad of nutrients, not much has been explored for serine and glycine. This work investigates the adaptation mechanisms of pancreatic CSCs to serine and glycine deficiency, focusing on metabolic plasticity and immune evasive properties. This study uses patient-derived xenografts, mRNA quantification, functional flow cytometry and biochemical assays to analyse these changes in their phenotype after deprivation. Key findings indicate an enhanced pluripotency altered immune evasion and reduced triglycerides uptake following deprivation adaptation. Single deprivation of serine or glycine increases overall epithelial-mesenchymal transition (EMT), while double deprivation reduces it, highlighting its complex metabolism. Our results also have implications for understanding the adaptation capabilities and strategies of cancer stem cells in scarce environments.<br /><br /> 000152831 521__ $$aGraduado en Biotecnología 000152831 540__ $$aDerechos regulados por licencia Creative Commons 000152831 691__ $$a0 000152831 692__ $$a 000152831 700__ $$aParejo Alonso, Beatriz$$edir. 000152831 700__ $$aSancho Andrés, Patricia$$edir. 000152831 7102_ $$aUniversidad de Zaragoza$$bBioquímica y Biología Molecular y Celular$$cBioquímica y Biología Molecular 000152831 7202_ $$aMoreno Loshuertos, Raquel$$eponente 000152831 8560_ $$f818785@unizar.es 000152831 8564_ $$s1298505$$uhttps://zaguan.unizar.es/record/152831/files/TAZ-TFG-2024-2345.pdf$$yMemoria (eng) 000152831 909CO $$ooai:zaguan.unizar.es:152831$$pdriver$$ptrabajos-fin-grado 000152831 950__ $$a 000152831 951__ $$adeposita:2025-04-01 000152831 980__ $$aTAZ$$bTFG$$cCIEN 000152831 999__ $$a20240610123327.CREATION_DATE