000152976 001__ 152976
000152976 005__ 20251017144550.0
000152976 0247_ $$2doi$$a10.1016/j.talanta.2025.128011
000152976 0248_ $$2sideral$$a143480
000152976 037__ $$aART-2025-143480
000152976 041__ $$aeng
000152976 100__ $$0(orcid)0000-0003-1654-3803$$aCamacho-Aguayo, Javier$$uUniversidad de Zaragoza
000152976 245__ $$aIn situ Metal-Enhanced Fluorescence of gold nanoclusters for enzymatic biosensors
000152976 260__ $$c2025
000152976 5203_ $$aA fluorescent enzymatic biosensor based on the in-situ observation of the MEF (Metal- Enhanced Fluorescence) effect has been developed for the first time. It has been demonstrated that during the enzymatic reaction of cadaverine with Diamine Oxidase (DAO) (used as proof of concept) in the presence of Au (III), the product can reduce the gold precursor to form AuNPs. When the reaction takes place in the presence of silica-coated gold nanoclusters (AuNCs@Si), an increase in the fluorescence signal is observed, which can also be related to the concentration of cadaverine. To understand the mechanism, the interaction between the AuNCs@Si and the enzymatic reaction, as well as their interaction with Au (III), has been studied. Under optimal conditions, the method shows a linear response range up to 250 μM Cadaverine, a limit of quantification of 52 μM and a limit of detection of 16 μM, which is about two orders of magnitude lower than the method without MEF (DAO/cadaverine/Au (III). It has been applied to the determination of cadaverine in spiked oily fish with a recovery of 95 ± 8 % (n = 3) and no significant differences (P = 0.05) were observed compared to a reference method. This MEF effect was also observed for the enzymatic determination of both xanthine with xanthine oxidase and tyramine with tyramine oxidase.
000152976 536__ $$9info:eu-repo/grantAgreement/ES/DGA/E25-23R$$9info:eu-repo/grantAgreement/ES/DGA-FEDER/Una manera de hacer Europa$$9info:eu-repo/grantAgreement/ES/MICINN/PID2022-139235OB-I00
000152976 540__ $$9info:eu-repo/semantics/closedAccess$$aby-nc-nd$$uhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
000152976 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000152976 700__ $$aNavarro Dominguez, Jesús
000152976 700__ $$aLambea, Daniel
000152976 700__ $$aFelices, Carlos
000152976 700__ $$0(orcid)0000-0002-7902-6005$$aMarcos Ruiz, Susana de$$uUniversidad de Zaragoza
000152976 700__ $$0(orcid)0000-0002-8973-5104$$aGalbán, Javier$$uUniversidad de Zaragoza
000152976 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica
000152976 773__ $$g292 (2025), 128011 [6 pp.]$$pTalanta$$tTalanta$$x0039-9140
000152976 8564_ $$s1984588$$uhttps://zaguan.unizar.es/record/152976/files/texto_completo.pdf$$yVersión publicada$$zinfo:eu-repo/date/embargoEnd/2026-04-03
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000152976 909CO $$ooai:zaguan.unizar.es:152976$$particulos$$pdriver
000152976 951__ $$a2025-10-17-14:11:35
000152976 980__ $$aARTICLE