000153051 001__ 153051
000153051 005__ 20251017144645.0
000153051 0247_ $$2doi$$a10.1016/j.vetmic.2019.02.023
000153051 0248_ $$2sideral$$a141784
000153051 037__ $$aART-2019-141784
000153051 041__ $$aeng
000153051 100__ $$0(orcid)0000-0001-7106-0068$$aGarza-Moreno, Laura
000153051 245__ $$aCharacterization of Mycoplasma hyopneumoniae strains in vaccinated and non-vaccinated pigs from Spanish slaughterhouses
000153051 260__ $$c2019
000153051 5060_ $$aAccess copy available to the general public$$fUnrestricted
000153051 5203_ $$aThis study aimed to describe Mycoplasma hyopneumoniae (M. hyopneumoniae) genetic variability in vaccinated (V) and non-vaccinated (NV) slaughtered pigs showing cranio-ventral pulmonary consolidation (CVPC). Ten V and 10 NV fattening farms with respiratory problems associated to M. hyopneumoniae were selected. Lung lesions of one batch per farm were scored at slaughterhouse and the enzootic pneumonia (EP)-index was calculated. Moreover, three lungs showing the most extensive CVPC per farm were sampled and tested for M. hyopneumoniae detection by real-time (rt)-PCR. Positive samples with cycle threshold ≤30 were selected to be genotyped by sequencing of four loci (P97, P146, H1 and H5). Typing profiles (TP) were assigned considering four or two (P97, P146) loci. Five commercial vaccines for M. hyopneumoniae (VS) and two reference strains (RF) were also genotyped. The EP-index (mean ± SD) in NV farms (3.8 ± 1.9) was not significantly different from V ones (2.2 ± 1.3). From the 60 selected lungs, 46 (76.7%) were M. hyopneumoniae positive by rt-PCR (25/30 and 21/30 from NV and V farms, respectively), and 43 (93.5%) of those were successfully genotyped. A total of 24 different TP(12 in V and 12 in NV farms) or 17 TP(9 in V and 9 in NV farms, being one TP in both farm types) were identified by analyzing four or two loci, respectively. One to three TP per farm were detected, being different from VS and RF. Interestingly, farms with same breeding origin had the same TP using two loci, but such link was not found using four loci. Therefore, high inter-farm and limited intra-farm M. hyopneumoniae genetic variability were detected, but variability depended on the number of studied loci.
000153051 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000153051 590__ $$a3.03$$b2019
000153051 591__ $$aVETERINARY SCIENCES$$b7 / 141 = 0.05$$c2019$$dQ1$$eT1
000153051 591__ $$aMICROBIOLOGY$$b64 / 133 = 0.481$$c2019$$dQ2$$eT2
000153051 592__ $$a1.138$$b2019
000153051 593__ $$aMedicine (miscellaneous)$$c2019$$dQ1
000153051 593__ $$aVeterinary (miscellaneous)$$c2019$$dQ1
000153051 593__ $$aMicrobiology$$c2019$$dQ2
000153051 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000153051 700__ $$aSegalés, Joaquim
000153051 700__ $$aAragón, Virginia
000153051 700__ $$aCorrea-Fiz, Florencia
000153051 700__ $$aPieters, Maria
000153051 700__ $$aCarmona, Marta
000153051 700__ $$aKrejci, Roman
000153051 700__ $$aSibila, Marina
000153051 773__ $$g231 (2019), 18-23$$pVet. microbiol.$$tVeterinary Microbiology$$x0378-1135
000153051 8564_ $$s249981$$uhttps://zaguan.unizar.es/record/153051/files/texto_completo.pdf$$yPostprint
000153051 8564_ $$s1174747$$uhttps://zaguan.unizar.es/record/153051/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000153051 909CO $$ooai:zaguan.unizar.es:153051$$particulos$$pdriver
000153051 951__ $$a2025-10-17-14:33:48
000153051 980__ $$aARTICLE