000153080 001__ 153080
000153080 005__ 20251017144555.0
000153080 0247_ $$2doi$$a10.1186/s13287-020-1573-6
000153080 0248_ $$2sideral$$a116480
000153080 037__ $$aART-2020-116480
000153080 041__ $$aeng
000153080 100__ $$aMartínez-Muriana, A.
000153080 245__ $$aCombined intramuscular and intraspinal transplant of bone marrow cells improves neuromuscular function in the SOD1G93A mice
000153080 260__ $$c2020
000153080 5060_ $$aAccess copy available to the general public$$fUnrestricted
000153080 5203_ $$aBackground: The simultaneous contribution of several etiopathogenic disturbances makes amyotrophic lateral sclerosis (ALS) a fatal and challenging disease. Here, we studied two different cell therapy protocols to protect both central and peripheral nervous system in a murine model of ALS. Methods: Since ALS begins with a distal axonopathy, in a first assay, we performed injection of bone marrow cells into two hindlimb muscles of transgenic SOD1G93A mice. In a second study, we combined intramuscular and intraspinal injection of bone marrow cells. Fluorescence-activated cell sorting was used to assess the survival of the transplanted cells into the injected tissues. The mice were assessed from 8 to 16 weeks of age by means of locomotion and electrophysiological tests. After follow-up, the spinal cord was processed for analysis of motoneuron survival and glial cell reactivity. Results: We found that, after intramuscular injection, bone marrow cells were able to engraft within the muscle. However, bone marrow cell intramuscular injection failed to promote a general therapeutic effect. In the second approach, we found that bone marrow cells had limited survival in the spinal cord, but this strategy significantly improved motor outcomes. Moreover, we also found that the dual cell therapy tended to preserve spinal motoneurons at late stages of the disease and to reduce microgliosis, although this did not prolong mice survival. Conclusion: Overall, our findings suggest that targeting more than one affected area of the motor system at once with bone marrow cell therapy results in a valuable therapeutic intervention for ALS.
000153080 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/SEV-2013-0317
000153080 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000153080 590__ $$a6.832$$b2020
000153080 591__ $$aCELL BIOLOGY$$b45 / 195 = 0.231$$c2020$$dQ1$$eT1
000153080 591__ $$aCELL & TISSUE ENGINEERING$$b7 / 29 = 0.241$$c2020$$dQ1$$eT1
000153080 591__ $$aMEDICINE, RESEARCH & EXPERIMENTAL$$b24 / 140 = 0.171$$c2020$$dQ1$$eT1
000153080 592__ $$a1.599$$b2020
000153080 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2020$$dQ1
000153080 593__ $$aMolecular Medicine$$c2020$$dQ1
000153080 593__ $$aMedicine (miscellaneous)$$c2020$$dQ1
000153080 593__ $$aCell Biology$$c2020$$dQ1
000153080 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000153080 700__ $$aPastor, D.
000153080 700__ $$aMancuso, R.
000153080 700__ $$0(orcid)0000-0002-2898-4561$$aRando, A.
000153080 700__ $$0(orcid)0000-0001-5687-6704$$aOsta, R.$$uUniversidad de Zaragoza
000153080 700__ $$aMartínez, S.
000153080 700__ $$aLópez-Vales, R.
000153080 700__ $$aNavarro, X.
000153080 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000153080 773__ $$g11, 1 (2020), [11 pp.]$$pStem cell res. ther.$$tStem cell research & therapy$$x1757-6512
000153080 85641 $$uhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85079083700&doi=10.1186%2fs13287-020-1573-6&partnerID=40&md5=e8fc8332122b68213dbc1e4a3daa5591$$zTexto completo de la revista
000153080 8564_ $$s4545519$$uhttps://zaguan.unizar.es/record/153080/files/texto_completo.pdf$$yVersión publicada
000153080 8564_ $$s2404209$$uhttps://zaguan.unizar.es/record/153080/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000153080 909CO $$ooai:zaguan.unizar.es:153080$$particulos$$pdriver
000153080 951__ $$a2025-10-17-14:12:47
000153080 980__ $$aARTICLE