000153185 001__ 153185
000153185 005__ 20251017144643.0
000153185 0247_ $$2doi$$a10.1021/acs.jproteome.4c00910
000153185 0248_ $$2sideral$$a143682
000153185 037__ $$aART-2025-143682
000153185 041__ $$aeng
000153185 100__ $$aCastelló-Ruiz, María
000153185 245__ $$aEffect of capacitation on proteomic profile and mitochondrial parameters of spermatozoa in bulls
000153185 260__ $$c2025
000153185 5203_ $$aSperm capacitation is a critical process for fertilization. This work aims to analyze the effect in vitro capacitation had on the proteome and mitochondrial parameters of bull spermatozoa. Viability, mitochondrial membrane potential (MMP), and reactive oxygen species (mROS) were assessed by flow cytometry in noncapacitated (NC) and in vitro capacitated (IVC) sperm. Proteome was evaluated using SWATH-MS. In vitro capacitation significantly induced a decrease in sperm viability, a high MMP, and an increase in mROS production. Within the group of living spermatozoa, the capacitation significantly induced a decrease in healthy mitochondrial spermatozoa, as well as an increase in mROS production, without affecting the MMP intensity. A total number of 72 differentially abundant proteins were found of which 63 were over-represented in the NC sperm group and 9 in the IVC sperm group. It was observed that many proteins associated with the sperm membrane and acrosome were lost during the capacitation process. For the IVC sperm, the functional enrichment was found in proteins related to the oxidative phosphorylation process. Our results indicate that the capacitation process induces a significant loss of seminal plasma-derived membrane proteins and a significant increase in proteins related with the oxidative phosphorylation (OXPHOS) pathway. Data are available via ProteomeXchange with identifiers PXD056424 and PXD042286.
000153185 536__ $$9info:eu-repo/grantAgreement/ES/MICINN-AEI/PRTR-C17.I1
000153185 540__ $$9info:eu-repo/semantics/closedAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000153185 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000153185 700__ $$aGacem, Sabrina
000153185 700__ $$aSánchez del Pino, Manuel M.
000153185 700__ $$aHidalgo, Carlos O.
000153185 700__ $$aTamargo, Carolina
000153185 700__ $$aÁlvarez-Rodríguez, Manuel
000153185 700__ $$0(orcid)0000-0001-5316-1703$$aYániz, Jesús L.$$uUniversidad de Zaragoza
000153185 700__ $$aSilvestre, Miguel A.
000153185 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal
000153185 773__ $$g24, 4 (2025), 1817-1831$$pJ. proteome res.$$tJOURNAL OF PROTEOME RESEARCH$$x1535-3893
000153185 8564_ $$s8675729$$uhttps://zaguan.unizar.es/record/153185/files/texto_completo.pdf$$yVersión publicada$$zinfo:eu-repo/date/embargoEnd/2026-03-25
000153185 8564_ $$s3083970$$uhttps://zaguan.unizar.es/record/153185/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada$$zinfo:eu-repo/date/embargoEnd/2026-03-25
000153185 909CO $$ooai:zaguan.unizar.es:153185$$particulos$$pdriver
000153185 951__ $$a2025-10-17-14:33:08
000153185 980__ $$aARTICLE