153185 20251219150012.0 doi 10.1021/acs.jproteome.4c00910 sideral 143682 ART-2025-143682 eng Castelló-Ruiz, María Effect of capacitation on proteomic profile and mitochondrial parameters of spermatozoa in bulls 2025 Sperm capacitation is a critical process for fertilization. This work aims to analyze the effect in vitro capacitation had on the proteome and mitochondrial parameters of bull spermatozoa. Viability, mitochondrial membrane potential (MMP), and reactive oxygen species (mROS) were assessed by flow cytometry in noncapacitated (NC) and in vitro capacitated (IVC) sperm. Proteome was evaluated using SWATH-MS. In vitro capacitation significantly induced a decrease in sperm viability, a high MMP, and an increase in mROS production. Within the group of living spermatozoa, the capacitation significantly induced a decrease in healthy mitochondrial spermatozoa, as well as an increase in mROS production, without affecting the MMP intensity. A total number of 72 differentially abundant proteins were found of which 63 were over-represented in the NC sperm group and 9 in the IVC sperm group. It was observed that many proteins associated with the sperm membrane and acrosome were lost during the capacitation process. For the IVC sperm, the functional enrichment was found in proteins related to the oxidative phosphorylation process. Our results indicate that the capacitation process induces a significant loss of seminal plasma-derived membrane proteins and a significant increase in proteins related with the oxidative phosphorylation (OXPHOS) pathway. Data are available via ProteomeXchange with identifiers PXD056424 and PXD042286. info:eu-repo/grantAgreement/ES/MICINN-AEI/PRTR-C17.I1 info:eu-repo/semantics/closedAccess All rights reserved http://www.europeana.eu/rights/rr-f/ info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Gacem, Sabrina Sánchez del Pino, Manuel M. Hidalgo, Carlos O. Tamargo, Carolina Álvarez-Rodríguez, Manuel Yániz, Jesús L. Universidad de Zaragoza (orcid)0000-0001-5316-1703 Silvestre, Miguel A. 2008 700 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Producción Animal 24, 4 (2025), 1817-1831 J. proteome res. JOURNAL OF PROTEOME RESEARCH 1535-3893 8675729 http://zaguan.unizar.es/record/153185/files/texto_completo.pdf Versión publicada info:eu-repo/date/embargoEnd/2026-03-25 3083970 http://zaguan.unizar.es/record/153185/files/texto_completo.jpg?subformat=icon icon Versión publicada info:eu-repo/date/embargoEnd/2026-03-25 oai:zaguan.unizar.es:153185 articulos driver 2025-12-19-14:58:21 ARTICLE