000153196 001__ 153196
000153196 005__ 20251017144552.0
000153196 0247_ $$2doi$$a10.1186/s13567-025-01512-0
000153196 0248_ $$2sideral$$a143697
000153196 037__ $$aART-2025-143697
000153196 041__ $$aeng
000153196 100__ $$aMarco Lorente, Paula A.$$uUniversidad de Zaragoza
000153196 245__ $$aDetection of prions in oocytes and ovaries of ewes naturally infected with classical scrapie
000153196 260__ $$c2025
000153196 5060_ $$aAccess copy available to the general public$$fUnrestricted
000153196 5203_ $$aClassical scrapie is a prion disease that affects goats and sheep and is transmitted mainly horizontally. However, infection can also occur vertically, from mother to offspring, but the exact mechanism is unclear. The possibilities include transmission during pregnancy, birth, or after birth. Some studies have reported that prions are present in various reproductive tissues and fluids, suggesting that vertical transmission could occur through the germ line. However, the presence of prions in female reproductive tissues and oocytes has not been extensively studied, so their role in vertical transmission is unknown. Oocytes and ovarian tissues from 25 naturally infected ewes (ARQ/ARQ and VRQ/VRQ genotypes) and healthy controls were analysed. The samples were subjected to PMCA for prion detection. Histopathological and immunohistochemical evaluations of ovarian tissues were also performed. We demonstrated that PrPSc can accumulate in the oocytes and ovaries of scrapie-infected ewes, indicating a potential germline route of transmission. Prion accumulation appears to be influenced by the host genotype and prion strain, emphasizing the need for ultrasensitive detection techniques. Further research under controlled conditions is necessary to elucidate the mechanisms and implications for disease control and breeding programs.
000153196 536__ $$9info:eu-repo/grantAgreement/EUR/INTERREG-POCTEFA/EFA 031-01-NEURO-COOP
000153196 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000153196 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000153196 700__ $$aZinkunegi, Maialen
000153196 700__ $$aSola, Diego$$uUniversidad de Zaragoza
000153196 700__ $$0(orcid)0009-0009-9836-7583$$aLarrañaga, Nerea$$uUniversidad de Zaragoza
000153196 700__ $$0(orcid)0000-0002-1590-3347$$aMarín, Belén$$uUniversidad de Zaragoza
000153196 700__ $$0(orcid)0000-0001-6629-6842$$aMoreno, Bernardino$$uUniversidad de Zaragoza
000153196 700__ $$0(orcid)0000-0002-7173-7216$$aBadiola, Juan J.$$uUniversidad de Zaragoza
000153196 700__ $$0(orcid)0000-0002-2746-3932$$aBolea, Rosa$$uUniversidad de Zaragoza
000153196 700__ $$0(orcid)0000-0001-9075-2764$$aOtero, Alicia$$uUniversidad de Zaragoza
000153196 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000153196 7102_ $$11003$$2027$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Anatom.Embriol.Humana
000153196 773__ $$g56, 1 (2025), 79 [9 pp.]$$pVet. res.$$tVeterinary Research$$x0928-4249
000153196 8564_ $$s1021493$$uhttps://zaguan.unizar.es/record/153196/files/texto_completo.pdf$$yVersión publicada
000153196 8564_ $$s2634645$$uhttps://zaguan.unizar.es/record/153196/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000153196 909CO $$ooai:zaguan.unizar.es:153196$$particulos$$pdriver
000153196 951__ $$a2025-10-17-14:12:09
000153196 980__ $$aARTICLE