000153205 001__ 153205
000153205 005__ 20251017144610.0
000153205 0247_ $$2doi$$a10.1016/j.ijbiomac.2021.01.206
000153205 0248_ $$2sideral$$a123162
000153205 037__ $$aART-2021-123162
000153205 041__ $$aeng
000153205 100__ $$0(orcid)0000-0003-1885-4365$$aOrtega-Alarcon, D.$$uUniversidad de Zaragoza
000153205 245__ $$aInfluence of the disordered domain structure of MeCP2 on its structural stability and dsDNA interaction
000153205 260__ $$c2021
000153205 5060_ $$aAccess copy available to the general public$$fUnrestricted
000153205 5203_ $$aMethyl-CpG binding protein 2 (MeCP2) is a transcriptional regulator and a chromatin-associated structural protein. MeCP2 deregulation results in two neurodevelopmental disorders: MeCP2 dysfunction is associated with Rett syndrome, while excess of activity is associated with MeCP2 duplication syndrome. MeCP2 is an intrinsically disordered protein (IDP) constituted by six structural domains with variable, small percentage of well-defined secondary structure. Two domains, methyl-CpG binding domain (MBD) and transcription repressor domain (TRD), are the elements responsible for dsDNA binding ability and recruitment of the gene transcription/silencing machinery, respectively. Previously we studied the influence of the completely disordered, MBD-flanking domains (N-terminal domain, NTD, and intervening domain, ID) on the structural and functional features of the MBD (Claveria-Gimeno, R. et al. Sci Rep. 2017, 7, 41, 635). Here we report the biophysical study of the influence of the remaining domains (transcriptional repressor domain, TRD, and C-terminal domains, CTDa and CTDß) on the structural stability of MBD and the dsDNA binding capabilities of MBD and ID. The influence of distant disordered domains on MBD properties makes it necessary to consider the NTD-MBD-ID variant as the minimal protein construct for studying dsDNA/chromatin binding properties, while the full-length protein should be considered for transcriptional regulation studies.
000153205 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B25-17R$$9info:eu-repo/grantAgreement/ES/DGA/E45-17R$$9info:eu-repo/grantAgreement/ES/ISCIII/CPII13-00017$$9info:eu-repo/grantAgreement/ES/ISCIII-ERDF-ESF/PI15-00663-Investing in your future$$9info:eu-repo/grantAgreement/ES/ISCIII-ERDF-ESF/PI18-00349-Investing in your future$$9info:eu-repo/grantAgreement/ES/MCIU-AEI-FEDER/BES-2017-080739$$9info:eu-repo/grantAgreement/ES/MCIU-AEI-FEDER/BFU2016-78232-P
000153205 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000153205 590__ $$a8.025$$b2021
000153205 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b46 / 297 = 0.155$$c2021$$dQ1$$eT1
000153205 591__ $$aPOLYMER SCIENCE$$b6 / 90 = 0.067$$c2021$$dQ1$$eT1
000153205 591__ $$aCHEMISTRY, APPLIED$$b8 / 72 = 0.111$$c2021$$dQ1$$eT1
000153205 592__ $$a1.1$$b2021
000153205 593__ $$aBiochemistry$$c2021$$dQ1
000153205 593__ $$aEconomics and Econometrics$$c2021$$dQ1
000153205 593__ $$aStructural Biology$$c2021$$dQ1
000153205 593__ $$aMolecular Biology$$c2021$$dQ1
000153205 593__ $$aEnergy (miscellaneous)$$c2021$$dQ1
000153205 594__ $$a11.6$$b2021
000153205 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000153205 700__ $$aClaveria-Gimeno, R.
000153205 700__ $$0(orcid)0000-0002-1232-6310$$aVega, S.
000153205 700__ $$aJorge-Torres, O.C.
000153205 700__ $$aEsteller, M.
000153205 700__ $$0(orcid)0000-0001-5664-1729$$aAbian, O.$$uUniversidad de Zaragoza
000153205 700__ $$0(orcid)0000-0001-5702-4538$$aVelazquez-Campoy, A.$$uUniversidad de Zaragoza
000153205 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000153205 773__ $$g175 (2021), 58-66$$pInt. j. biol. macromol.$$tInternational journal of biological macromolecules$$x0141-8130
000153205 8564_ $$s1359722$$uhttps://zaguan.unizar.es/record/153205/files/texto_completo.pdf$$yVersión publicada
000153205 8564_ $$s2780766$$uhttps://zaguan.unizar.es/record/153205/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000153205 909CO $$ooai:zaguan.unizar.es:153205$$particulos$$pdriver
000153205 951__ $$a2025-10-17-14:16:56
000153205 980__ $$aARTICLE