000153218 001__ 153218 000153218 005__ 20251017144635.0 000153218 0247_ $$2doi$$a10.1038/s43586-023-00199-x 000153218 0248_ $$2sideral$$a133285 000153218 037__ $$aART-2023-133285 000153218 041__ $$aeng 000153218 100__ $$aBastos, Margarida 000153218 245__ $$aIsothermal titration calorimetry 000153218 260__ $$c2023 000153218 5060_ $$aAccess copy available to the general public$$fUnrestricted 000153218 5203_ $$aIsothermal titration calorimetry (ITC) has become the gold standard for studying molecular interactions in solution. Although it is increasingly being used in the soft matter and synthetic chemistry fields, ITC is most widely used for characterizing molecular interactions between ligands and macromolecules. This Primer starts by presenting the technique’s foundations and instrumentation, including a brief description of the standard assay, followed by a review of common applications. Further extensions and modifications of the technique are explored. These adaptations enable key features to be studied, such as cooperative effects associated with complex biological interactions and their regulation, alongside applications to other fields, including partition to membranes, kinetics and soft matter. Advantages and caveats in ITC are discussed, with a focus on best practices, instrument calibration, experimental design, data analysis and data reporting, as well as recent and future developments. 000153218 536__ $$9info:eu-repo/grantAgreement/EUR/COST/CA15126-MOBIEU 000153218 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/ 000153218 592__ $$a12.294$$b2023 000153218 593__ $$aMedicine (miscellaneous)$$c2023$$dQ1 000153218 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2023$$dQ1 000153218 594__ $$a46.1$$b2023 000153218 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion 000153218 700__ $$0(orcid)0000-0001-5664-1729$$aAbian, Olga$$uUniversidad de Zaragoza 000153218 700__ $$aJohnson, Christopher M. 000153218 700__ $$aFerreira-da-Silva, Frederico 000153218 700__ $$0(orcid)0000-0002-1232-6310$$aVega, Sonia 000153218 700__ $$0(orcid)0000-0003-4726-7821$$aJimenez-Alesanco, Ana$$uUniversidad de Zaragoza 000153218 700__ $$0(orcid)0000-0003-1885-4365$$aOrtega-Alarcon, David 000153218 700__ $$0(orcid)0000-0001-5702-4538$$aVelazquez-Campoy, Adrian$$uUniversidad de Zaragoza 000153218 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole. 000153218 773__ $$g3 (2023), 17$$pNat. rev. methods primers$$tNature reviews methods primers$$x2662-8449 000153218 8564_ $$s1023269$$uhttps://zaguan.unizar.es/record/153218/files/texto_completo.pdf$$yPostprint 000153218 8564_ $$s2090076$$uhttps://zaguan.unizar.es/record/153218/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint 000153218 909CO $$ooai:zaguan.unizar.es:153218$$particulos$$pdriver 000153218 951__ $$a2025-10-17-14:28:49 000153218 980__ $$aARTICLE