153229 20251009133840.0 doi 10.1007/s11259-025-10724-w sideral 143714 ART-2025-143714 eng Marteles, Diana Universidad de Zaragoza Impact of single versus multiple infection on serum protein fractions in cats 2025 Access copy available to the general public Unrestricted Serum protein electrophoresis (SPE) is a widely used diagnostic tool for identifying acute and chronic inflammation, as well as immunodeficiencies. However, the impact of co-infections on SPE patterns in cats remains poorly understood. This study explored the utility of SPE in differentiating immune responses between cats infected with a single pathogen and those with multiple co-infections. A total of 79 serum samples from stray European Shorthair cats in Zaragoza, Spain, were analyzed. Fifty cats had a single infection, while 29 were co-infected with 2–4 pathogens. Agarose gel electrophoresis was used to assess protein profiles and statistical analyses were performed to identify significant differences between groups. The results showed no major differences in protein profiles between single and co-infected cats, with polyclonal hypergammaglobulinemia being most common in single-pathogen infections. Therefore, these findings indicate that SPE may have limitations in distinguishing between single and multiple infections in cats, contrasting with some previous studies. info:eu-repo/grantAgreement/ES/DGA/AR15-23R info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es info:eu-repo/semantics/conferenceObject info:eu-repo/semantics/publishedVersion Lebrero, María Eugenia Universidad de Zaragoza (orcid)0000-0001-5816-6167 Fernández, Antonio Universidad de Zaragoza (orcid)0000-0002-2557-4890 Ortín, Aurora Universidad de Zaragoza (orcid)0000-0002-0313-5926 González, Ana Universidad de Zaragoza (orcid)0000-0002-8727-0323 Morell, Carmen Villanueva, María Jesús Schäfer, Ingo Quílez, Pablo Universidad de Zaragoza (orcid)0009-0003-9757-3840 Verde, Maite Universidad de Zaragoza (orcid)0000-0003-2957-1379 Gómez, Alex Universidad de Zaragoza (orcid)0000-0002-9723-9004 Villanueva-Saz, Sergio Universidad de Zaragoza (orcid)0000-0001-6209-4282 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 49, 3 (2025), 158 [5 pp.] Vet. res. commun. Veterinary Research Communications 0165-7380 843593 http://zaguan.unizar.es/record/153229/files/texto_completo.pdf Versión publicada 2399985 http://zaguan.unizar.es/record/153229/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:153229 articulos driver 2025-10-09-13:25:51 ARTICLE