000153607 001__ 153607
000153607 005__ 20251017144551.0
000153607 0247_ $$2doi$$a10.1177/03009858251331106
000153607 0248_ $$2sideral$$a143757
000153607 037__ $$aART-2025-143757
000153607 041__ $$aeng
000153607 100__ $$0(orcid)0000-0002-9723-9004$$aGómez, Álex$$uUniversidad de Zaragoza
000153607 245__ $$aClinicopathological progression and molecular characterization of intestinal dilatation syndrome in commercial brown layers
000153607 260__ $$c2025
000153607 5060_ $$aAccess copy available to the general public$$fUnrestricted
000153607 5203_ $$aIntestinal dilatation syndrome (IDS) is a poorly described condition affecting layers and breeder hens globally. Its prevalence is increasing, particularly in free-range systems, but the cause remains unknown. This retrospective study examined 35 hens from 3 flocks: free-range flock A (n = 20) and enriched-caged flock B (n = 5), both affected by IDS, and enriched-caged flock C (n = 10), with no history of IDS. Clinicopathological studies were performed on these hens, and metagenomic analysis was conducted on the proventriculus and jejunum of hens from flock A (n = 2) and flock C (n = 2). Based on clinical signs and lesions, 3 progressive stages of IDS were identified. In the first stage, although hens were without clinical signs, proventricular dilatation and lymphoplasmacytic and heterophilic jejunitis and duodenitis were observed. The second stage was marked by cachexia, pale and small combs and wattles, and severe egg production drop. Jejunal dilatation was observed, with microscopic evidence of necrotic, lymphoplasmacytic and heterophilic jejunitis; ganglioneuritis; and mineralization of the jejunal nervous plexuses and subserosal ganglia. In the third stage, spontaneous death occurred due to jejunal volvulus and vascular involvement. Affected hens (stage 2) also exhibited elevated cloacal temperatures (>0.9°C) and marked heterophilia. Metagenomic analysis identified sequences consistent with Megrivirus C in IDS-affected hens and a disruption of the gut microbiota, with increased abundance of Fusobacterium mortiferum and Megamonas funiformis. In conclusion, this study describes in detail the clinicopathological progression of the IDS and suggests that Megrivirus C, in combination with opportunistic intestinal bacteria, could play a role in the pathogenesis of this disease.
000153607 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
000153607 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000153607 700__ $$0(orcid)0000-0002-2685-1730$$aRodríguez-Largo, Ana
000153607 700__ $$aPérez, Estela
000153607 700__ $$aGarcía Freire, Serafín
000153607 700__ $$aHundehege, Christiane
000153607 700__ $$aBerberich, Eva
000153607 700__ $$0(orcid)0000-0002-2053-9842$$aLuján, Lluís$$uUniversidad de Zaragoza
000153607 700__ $$aCortés, Diego$$uUniversidad de Zaragoza
000153607 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000153607 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000153607 773__ $$g(2025), [8 pp.]$$pVet. pathol.$$tVeterinary Pathology$$x0300-9858
000153607 8564_ $$s890415$$uhttps://zaguan.unizar.es/record/153607/files/texto_completo.pdf$$yPostprint
000153607 8564_ $$s710981$$uhttps://zaguan.unizar.es/record/153607/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000153607 909CO $$ooai:zaguan.unizar.es:153607$$particulos$$pdriver
000153607 951__ $$a2025-10-17-14:11:48
000153607 980__ $$aARTICLE