000153661 001__ 153661
000153661 005__ 20251017144606.0
000153661 0247_ $$2doi$$a10.1016/j.theriogenology.2025.117428
000153661 0248_ $$2sideral$$a143790
000153661 037__ $$aART-2025-143790
000153661 041__ $$aeng
000153661 100__ $$0(orcid)0000-0003-3929-5064$$aCarvajal-Serna, M.
000153661 245__ $$aMelatonin supplementation does not improve ovine pre- or post-hatching development in vitro
000153661 260__ $$c2025
000153661 5203_ $$aMelatonin plays a crucial role in the regulation of reproductive seasonality in sheep. Melatonin supplementation has been widely used both in vivo to increase fertility and prolificacy, and in vitro during oocyte maturation, fertilization, and embryo culture. However, its effects have only been assessed in vitro up to the hatched blastocyst stage in conventional embryo culture systems. This study aimed to evaluate the effects of melatonin supplementation from oocyte in vitro maturation (IVM) through post-hatching embryonic stages in vitro on the development of the first three embryonic lineages. Supplementation with melatonin at 10−8 or 10−6 M during IVM, in vitro fertilization (IVF), and in vitro culture (IVC) did not affect cleavage and blastocyst rates at day (D) 8, nor embryo survival and growth at D12. While hypoblast development was not affected, epiblast survival was reduced in D12 embryos treated with 10−6 M melatonin from the blastocyst stage onward. In conclusion, melatonin supplementation did not show a clear beneficial effect on ovine in vitro embryo production or lineage development during post-hatching embryo culture.
000153661 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/ECQ2018-005184-P$$9info:eu-repo/grantAgreement/ES/MICINN/PID2021-122153NA-I00
000153661 540__ $$9info:eu-repo/semantics/closedAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000153661 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000153661 700__ $$aMartínez de los Reyes, N.
000153661 700__ $$aMarigorta, P.
000153661 700__ $$aBermejo-Álvarez, P.
000153661 700__ $$aRamos-Ibeas, P.
000153661 773__ $$g241 (2025), 117428 [7 pp.]$$p1879-3231]$$tTHERIOGENOLOGY$$x0093-691X
000153661 8564_ $$s4038889$$uhttps://zaguan.unizar.es/record/153661/files/texto_completo.pdf$$yVersión publicada
000153661 8564_ $$s2489309$$uhttps://zaguan.unizar.es/record/153661/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000153661 909CO $$ooai:zaguan.unizar.es:153661$$particulos$$pdriver
000153661 951__ $$a2025-10-17-14:15:23
000153661 980__ $$aARTICLE